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Frequently Asked Questions

Frequently Asked Questions

 

Select a category or product below for shortcuts to answers:

ATP Products:  UltraSnap, AquaSnap, SuperSnap, SnapShot, WaterShot
Monitoring Systems    EnSURE, SystemSURE Plus, Pi-102
Microorganism Tests:  MicroSnap TVCMicroSnap ColiformMicroSnap E. coliInSite Listeria 
Allergen Prevention Tests: AllerSnap 
Surface Residue Tests:
ProClean, SpotCheck Plus 
Processing Efficiency Tests:
CrossCheck ACP, ZymoSnap ALP
Sample Collection Devices:  Q-Swab, Transport Swab, Sponges, QD-Loop
SureTrend Software   
Other questions   

  Do you have a question that is not answered below? Ask your question here. 

 

ATP Products

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UltraSnap, SuperSnap, AquaSnap 

How does bioluminescence work?

Bioluminesence is the result of a biochemical reaction and is the science behind Hygiena ATP detection tests. The reaction includes the following elements:

  • Luciferase enzyme- naturally occuring in fireflies, or synthetically manufactured
  • Adenosine triphosphate (ATP) - the energy moleclule of all living organisms
  • Oxygen- a catalyst
  • Luicferin- a molecule that undergoes a chemical charge when affixed by an enzyme

ATP + Luciferin + Luciferase + O2 == == Light output

The reaction occures in two steps: The substrate combines with ATP and oxygen, which is controlled by the enzyme. The chemical energy in step 1 excites a specific molecule (the combination of Luciferin and Luciferase). The result is decay which is manifested as photon emission, or light production. The light is simply a by-product of the chemical reaction and does not depend on light.

Biolum reaction 

What is ATP Monitoring?

ATP monitoring is a rapid testing method used by food and beverage processors to quickly assess the cleanliness of surfaces or liquid samples from such places as CIP systems. Adenosine Triphosphate (ATP) is present in all organic material and is the universal unit of energy used in all living cells. ATP is produced and/or broken down in metabolic processes in all living systems. Processes such as photosynthesis in plants, muscle contraction in humans, respiration in fungi, and fermentation in yeast are all driven by ATP. Therefore, most foods and microbial cells will contain some level of naturally occurring ATP.

Hygiena luminometers (in conjunction with ATP swabs) use bioluminescence to detect residual ATP as an indicator of surface cleanliness. The presence of ATP on a surface indicates improper cleaning and the presence of contamination, including food residue, allergens and/or bacteria. This implies a potential for the surface to harbor and support bacterial growth.

ATP monitoring is used in food and beverage facilities to confirm that ATP presence is eliminated or minimized by effective sanitation procedures. ATP monitoring prevents cross-contamination, ensures product integrity, potentially improves product shelf life, protects brand reputation, and complies with GMP standards and HACCP requirements.

 

How many food and beverage processing companies are doing ATP monitoring?

Nearly all food and beverage companies that process food with any water activity or can sustain organisms do ATP testing. Companies with highly processed materials like oil or companies dealing with products with no water activity like dry grains, sometimes will not do ATP testing. ATP testing is used around the globe and is a recognized tool by auditors for validating cleaning processes and complies with government and HACCP regulations.

 

Do all foods contain ATP?

Because ATP is present in all organic matter, all foods contain ATP at varying levels. This is why testing for ATP presence is the preferred way to measure the cleanliness of surfaces and water samples. Highly processes foods such as oil will have very little to no ATP.

 

Are there any studies or white papers on ATP testing?

Many manufacturers offer seemingly scientific research supporting their own ATP systems. It is important for customers to understand that only objective studies conducted by independent laborites should be accepted as scientific evidence of product performance. Silliker Laboratories, the leading international food testing and safety laboratory, conducted an independent study comparing five commercial ATP systems. This study found that Hygiena’s products offer superior linearity, sensitivity, repeatability, and accuracy. Request a copy here!

What is an RLU?

RLU stands for Relative Light Unit and is the unit of measure used in bioluminescence. When a test swab is activated, a bioluminescent reaction occurs, generating light output. Luminometers measure and quantify that light with an RLU output. Because manufacturers use different sensor technologies and algorithms for adding up the photons, RLU measurements will vary from system to system. However, because the ATP bioluminescence reaction is linear, the more ATP present means the more light will be present. This makes comparing systems easy. (Comparing RLU values is like comparing Fahrenheit and Celsius; they are two different scales for the same temperature.) For more information on comparing systems, click here.

The relationship between the amount of ATP on the sample and the RLU result reading on the luminometer is simple:

High contamination (improper cleaning) = Large amount of ATP = More light produced in reaction= High RLU reading on SystemSURE Plus or EnSURE

 

graph 1 passcautionfail

 

The RLU reading is directly proportional to the amount of ATP collected from the sample. A high RLU reading indicates a large amount of ATP at the test location. This in turn indicates improper cleaning and the presence of contaminants.

Cleaning properly results in less ATP at the location. Less ATP results in less light output during the bioluminescent reaction and consequently, a lower RLU reading.

 

Does a 0 (zero) RLU result indicate sterility?

Sterile is a term relating to a true microbiology test i.e., the absence of living bacteria. An ATP test detects all sources of ATP but cannot differentiate ATP from food or bacteria, thus a zero RLU result cannot be equated with sterility.

 

What is the limit of detection of Hygiena ATP tests?

The limit of detection depends on the test device and luminometer used:

  • UltraSnap & SystemSURE Plus : 1 femtomole of ATP
  • SuperSnap & SystemSURE Plus : <0.20 femtomoles of ATP
  • SuperSnap & EnSURE : <0.10 femtomoles of ATP

 

How do I set my Pass and Fail limits for my ATP program?

All systems come preset with generic limits that can usually be applied to most processing facilities. These thresholds have been established over the years by companies doing ATP testing and supported by research papers on the science. These thresholds are typically a good starting point for companies new to ATP testing.

Click here for a video and step by step guide for setting up and determining RLU limits!

 

Can I have different limits for different locations?

Yes. Pass/Fail limits are 100% customizable to fit your facility and program needs. To adjust limits for specific locations, simply edit the Unit Set-Up data in the SureTrend software. 

 

Can I compare RLU measurement to CFU plate counts?

ATP systems with standard ATP test swabs detect the presence of ATP and cannot distinguish microbial ATP from other organic ATP left behind after cleaning. RLU results could potentially be microbial cells, organic ATP from product residue, or both. This makes comparing RLU to CFU difficult. Studies have shown that there is anywhere between 60-90% correlation between RLU and CFU readings depending on the environment in which the system is used.

The presence of ATP on the surface indicates that it has not been adequately cleaned. The primary purpose of cleaning is to remove product residue for product contact surfaces. Effective cleaning simultaneously removes the material capable of supporting microbial survival and growth, as well as many of the microbes themselves. The technology behind ATP systems has been changing quite rapidly over the past few years and now there are ATP systems that can run tests specifically for microorganisms.

For more information on these multifunctional ATP systems see the MicroSnap platform from Hygiena. 

 

 

What is a biofilm?

A biofilm is formed when microorganisms find a receptive environment where they are exposed to food and moisture. The microorganisms work together as a population and secrete a sticky polymer to form a solid matrix attached to a surface. Once a biofilm is established, it is very difficult to eliminate because the microbes are reinforced and protected by the matrix, making them very resistant to sanitizers. Biofilms are often responsible for poor product quality and/or lost product due to contamination, causing costly damage to both product and equipment. The threat of a biofilm can be eliminated with proper ATP hygiene monitoring, allowing early detection and removal of food residue – thus eliminating the food source for possible biofilm-forming microbes. In addition, Hygiena ATP swabs have a unique detergent on the swab tip that cuts through biofilm and exposes the underlying cells. If a biofilm has already developed, there will be more ATP on a surface, which will result in a higher RLU result.

biofilm-diagram

 

When should I do ATP testing?

ATP testing should ideally be done after cleaning, but before sanitization. Because sanitizers are less effective when product residues are on the surface, it’s best to eliminate all ATP before the sanitization step. In some facilities, testing after cleaning is not possible. In these scenarios testing after sanitization is acceptable. Most sanitizers will not affect Hygiena’s ATP test devices and if the proper concentration and dwell time is followed there should be no interference from sanitizers.

Clean test Sanitize 

What is the difference between cleaning, disinfection, and sterilization?

Cleaning is the removal of organic matter and the reduction of risk from material which is a potential contaminant, or material which could support the survival and growth of microbes. 

Disinfection  is the reduction of microbiological hazards to a minimal level but not necessarily complete inactivation of all microbiological hazards. 

Sterilization is the complete destruction and inactivation of all microbial hazard. 

 

What areas should be tested?

Food contact areas (direct and indirect) and hard-to-clean areas should be the main focus of your swabbing program. Direct contact areas are surfaces where the presence of any contaminant will taint the final product. Indirect contact areas are those where splashed product, dust, or liquid has the potential to be dropped, drained, or transferred onto the product. Hard-to-clean areas may include filler heads, O-rings, nozzles, and areas with irregularly-shaped surfaces, corners, grooves and cracks.

 

What is the proper swabbing technique?

You should swab an area that is about 4 by 4 square inches (10 x 10 cm) – or in the case of a hard-to-clean area, as much of the surface as possible. Do not let the swab come into contact with anything other than the test area to avoid contamination. Apply pressure to the swab to pick up surface residue and penetrate any biofilm that may be present. After collecting the sample, place the swab back in swab tube. Once the device has been activated, it should be read as soon as possible

Correct         Incorrect
proper swabbing   wrong swabbing

Sufficient pressure to create flex in
the swab shaft

Do not touch the swab shaft

Rotate to collect sample on all sides
of swab tip

 

Only lightly swabbing the surface

Touching the swab shaft with finger

Only collecting sample on one side 
of swab tip

 

  

How long are ATP tests good for?

ATP test devices have a 15 month shelf life at refrigerated temperatures (2-8° C) and 4 week shelf life at room temperature (21-25° C). All swab labels are printed with expiration month and year for your convenience.

 

Does a surface have to be dry before I can swab?

Surfaces do not have to be dry to perform a swab test. However, for consistent readings, surfaces should be swabbed in the same conditions (always wet or always dry). Hygiena swabs come premoistened for maximum sample recovery on dry surfaces.

 

How do sanitizers or soap affect Hygiena products?

Several sanitizers commonly used in the food and beverage industry have been tested at normal working strength and found to have no significant effect on either Snapshot or Ultrasnap performance. Only acid-based sanitizer, if used at higher than normal concentrations, have been found to have an effect on performance. If you are unsure about the chemical nature of your sanitizers, consult your sanitizer manufacturer or Hygiena technical support. 

 

Will direct sunlight affect test results?

Yes. Tests activated and measured in direct sunlight will distort results, increasing the RLU output. If a test must be collected outside, the swab device should be activated and measured out of the direct sunlight.

 

How does temperature affect ATP test result?

Ambient temperature of 20–22°C (70–72°F) is the temperature that provides optimal performance. The only time ambient temperatures can affect results is if reagents are at a low temperature. This can happen if testing is done immediately after taking the tests out of the refrigerator. If testing in cold environments the instrument will self-adjust to external temperature but the luciferase reagents needs to be at  20–22°C (70–72°F) to function best.

If reagents are cold, then the reaction will be slower and the RLU result for a given amount of ATP will be lower.

Temperature does not affect light measurement of the instrument provided that the instrument has equilibrated to the environmental conditions. The instrument will sense an environmental temperature change of 5°C and it will automatically initiate a 15 second recalibration sequence.

The user can also initiate a manual recalibration at any time by depressing and holding the OK button down for 3 seconds.

The system can be used in cold environments (e.g. 5°C) if the instrument is equilibrated for 10 – 15 minutes before use and that the reagent swab devices are kept warm e.g. by storage in an internal pocket close to body heat.

 

 

end faq

 

 

SnapShot, WaterShot ↑ Back to top 

 

Do I have to change my RLU limits to use SnapShot or WaterShot?

No. SnapShot and WaterShot work just like the device they are replacing, so you may continue testing the same way. However, superior design and reagent technology make SnapShot and WaterShot more consistent, reliable, and affordable. Since each manufacturer's test is a little different, we have made a unique version of SnapShot for each of the systems out there. To understand how we did this, it is helpful to understand how ATP bioluminescence reaction works. The graph below shows that as the amount of ATP goes up, so does RLU. It is a linear reaction on all instruments.

graph 1 passcautionfail

The next graph shows how SnapShot gives the same readings as the one it is replacing. Different dilutions of ATP were pipetted onto an ATP test. The results are plotted on a line. Then the slope of the line is determined. SnapShot is then designed to have the same results and slope. 

graph 2 slope

 

Why is SnapShot so much less expensive that the test I currently use?

Snapshot's design and Hygiena's manufacturing expertise allows us to charge less for SnapShot. In addition, the patented design also gives some added benefits. To better understand how the Snapshot design equates to greater cost savings, let us compare the Charm's PocketSwab™, BioControl's MVP and 3M's CleanTrace test to Hygiena's SnapShot. SnapShot uses 50% less plastic than other ATP tests and the Snap-Valve design eliminates the need for additional compartments that require foil seals. This reduces raw costs, production costs and is more environmentally friendly. 

cost effective parts hygiena2

 

Are there any studies or white papers proving SnapShot works better than manufacturers swabs?

A leading international food testing and safety laboratory conducted an independent study comparing five commercial ATP systems. The study incorporated SnapShot with other manufacturer systems and found that Hygiena’s products offer superior linearity, sensitivity, repeatability, and accuracy. For more information, or a copy of the full report, click here.

How does SnapShot give more reproducible results?

There are two reasons that SnapShot gives more reproducible results:

1. SnapShot uses Hygiena's unique liquid-stable reagent. By formulating the luciferin/luciferase and buffer together in a liquid-stable format, we are able to eliminate additional steps that add variability such as freeze-drying of enzymes and a reconstitution step when the test is activated. The histograms below show the percent variation of 60 tests with the same ATP concentration. Tests were run on two systems and then run with SnapShot. 

GRAPH4

 

2. The patented design of SnapShot allows the luciferin/luciferase enzyme, buffer, and contamination collected by the swab bud to mix without having to puncture foil-sealed chambers that may cause loss of sample. The diagram below shows the process that takes place when a test device with foil seals compared to SnapShot, without foil seals.

charmchambers 

Clean trace chambers 

snapshot chambers

 

How do I evaluate SnapShot against the current ATP device I am using?

Hygiena provides Side by Side evaluation kits that allow you to compare ATP test devices in a controlled environment. Doing a side by side comparison with this kit should not take more than 30 minutes. To request a Side by Side evaluation kit, contact Hygiena or a qualified distributor and request one of the following kits:

  • SBS-CH1616N - Charm
  • SBS-SBC1575 - BioControl
  • SBS-SPXL1333 - 3M/Biotrace

Hygiena makes SnapShot test for the following luminometers. It is important to note that some luminometers require a retrofit cap to allow SnapShot to work correctly.

Luminometers -Catalog numbers:

  • BioControl
    • Lightning MVP - SBC1575 BioControl Lightning MVP virtual demo
    • Lightning - SBC1575 BioControl Lightning virtual demo
  • 3M / Biotrace
    • Uni-Lite® NG - SPXL1333 3M / Biotrace NG virtual demo
    • Uni-Lite® XCEL - SPXL1333 3M / Biotrace XCEL virtual demo
    • Uni-Lite® - SPXL1333
    • Uni-Lite® NG Junior - SPXL2020
  • Charm
    • NovaLum™ - CH1616N
    • FireFly2 - CH1616N
    • LUM-T™ - CH1616N Charm LUMinator T virtual demo
    • LUMGiene™ - CH1616N
    • Firefly™ - CH1616N Charm FireFly virtual demo
    • LUM-K™ - CH1616N
  • Merck
    • Hy-Lite™ - SHL1414
  • Celsis
    • Lumac M™ - SS1211
    • SystemSURE - SS1211  

 

What is the proper swabbing technique with SnapShot swabs?

You should swab an area that is about 4 by 4 square inches (10 x 10 cm) – or in the case of a hard-to-clean area, as much of the surface as possible. Do not let the swab come into contact with anything other than the test area to avoid contamination. Apply pressure to the swab to pick up surface residue and penetrate any biofilm that may be present. After collecting the sample, place the swab back in swab tube. Once the device has been activated, it should be read as soon as possible.

Correct         Incorrect
proper swabbing   wrong swabbing

Sufficient pressure to create flex in
the swab shaft

Do not touch the swab shaft

Rotate to collect sample on all sides
of swab tip

 

Only lightly swabbing the surface

Touching the swab shaft with finger

Only collecting sample on one side 
of swab tip

 

  

How long are SnapShot and WaterShot tests good for?

ATP test devices have a 15 month shelf life at refrigerated temperatures (2-8° C) and 4 week shelf life at room temperature (21-25° C). All swab labels are printed with expiration month and year for your convenience.

 

Does a surface have to be dry before I can swab?

Surfaces do not have to be dry to perform a swab test. However, for consistent readings, surfaces should be swabbed in the same conditions (always wet or always dry). Hygiena swabs come premoistened for maximum sample recovery on dry surfaces.

 

How do sanitizers or soap affect Hygiena products?

Several sanitizers commonly used in the food and beverage industry have been tested at normal working strength and found to have no significant effect on either Snapshot or Ultrasnap performance. Only acid-based sanitizer, if used at higher than normal concentrations, have been found to have an effect on performance. If you are unsure about the chemical nature of your sanitizers, consult your sanitizer manufacturer or Hygiena technical support. 

 

Will direct sunlight affect test results?

Yes. Tests activated and measured in direct sunlight will distort results, increasing the RLU output. If a test must be collected outside, the swab device should be activated and measured out of the direct sunlight.

 

How does temperature affect ATP test result?

Ambient temperature of 20–22°C (70–72°F) is the temperature that provides optimal performance. The only time ambient temperatures can affect results is if reagents are at a low temperature. This can happen if testing is done immediately after taking the tests out of the refrigerator. If testing in cold environments the instrument will self-adjust to external temperature but the luciferase reagents needs to be at  20–22°C (70–72°F) to function best.

If reagents are cold, then the reaction will be slower and the RLU result for a given amount of ATP will be lower.

Temperature does not affect light measurement of the instrument provided that the instrument has equilibrated to the environmental conditions. The instrument will sense an environmental temperature change of 5°C and it will automatically initiate a 15 second recalibration sequence.

The user can also initiate a manual recalibration at any time by depressing and holding the OK button down for 3 seconds.

The system can be used in cold environments (e.g. 5°C) if the instrument is equilibrated for 10 – 15 minutes before use and that the reagent swab devices are kept warm e.g. by storage in an internal pocket close to body heat.

 

 

 

end faq

 

 

Monitoring Systems

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EnSURE, SystemSURE Plus, Pi-102

Does the system need to be calibrated?

Hygiena ATP systems self-calibrate at start up to ensure accurate readings. To further verify calibration, we offer a calibration control kit. Though the kit is not necessary for the performance of the system, a documented calibration process shows due diligence to auditors. With advanced technology, Hygiena systems will stay in calibration for the life of the device.

 

Are there maintenance costs I should be worried about?

Unlike other systems on the market, Hygiena’s EnSURE and SystemSURE Plus do not require expensive yearly maintenance or service contracts. Monitoring systems come with a 1 year warranty. Should you system become damaged or non-operational, Hygiena will promptly replace your device with a loaner so that you do not experience any interruption in your testing program.

 

Does the system do anything else besides ATP testing?

In addition to surface and water ATP testing, Hygiena’s EnSURE measures test swabs for:

 

How do I erase the results off the handheld?

There are two ways to erase the results off the handheld.

  1. If you are using SureTrend software, simply plug connect your luminometer to your computer, click "Synchronize" in SureTrend, upload results, and click "Yes" when asked if you would like to erase test results memory.
  2. If you want to erase the results without syncing to SureTrend, select the * button on the meter, then use the arrows to navigate to the Memory menu. Press "OK" to select, and then press and hold down the * button for 2 seconds. This will display the total number of stored results to be erased. To accept and start the erase function, press and hold down the "OK" button for 1 second. To cancel, press any other button.

 

Why do EnSURE and SystemSURE Plus come pre-programmed with Pass/Fail limits of 10 and 30?

The pre-programmed limits for food and beverage facilities are based on years of data, experience, and third part studies. Hygiena recommends that users validate these recommendations and adjust them to meet each facility's unique needs. To see the data or learn more, download this ATP Thresholds Technical Document.

 

I'm getting lower than expected results. What is wrong?

Almost all unexpected results can be resolved by asking yourself these questions:

  1. Have my swabs been stored at the correct temperatures and are within the expiration date? Swabs that have been stored incorrectly for prolonged periods of time may not perform as expected.  
  2. Am I holding the meter upright when taking measurement? Holding the meter upright ensures that the sensor at the bottom of the machine can see and quantify the light reaction accurately.
  3. Am I overloading the swab? Swabbing too larger of an area may result in picking up physical debris. This debris will inhibit the light reaction, giving lower than expected results.
  4. Is the read chamber dirty? A dirty read chamber could also block light from reaching the sensor. To check your read chamber, open the lid of your system, and use the vertical plastic tab to pull the read chamber out of the meter. (You may also find that pushing your pinky finger into the chamber, then lifting it out works easier for you.) If you see any water spots or debris on the clear plastic tip of the read chamber, you may wash the read chamber with warm water and let it air dry. Ensure the chamber is completely dry before replacing in the meter. If the read chamber has physical damage like cracks, or scratches on the clear plastic tip, contact customer service to order a replacement read chamber. 

If these tips do not resolve the problem, contact technical support.

 

I accidentally placed a wet swab into the read chamber. How can I clean the instrument?

One of the great features of Hygiena's EnSURE and SystemSURE Plus is the removable read chamber that can be hand washed in the event of situations like these. To remove the read chamber, open the lid of the system, and insert your finger into the read chamber, and pull it out. The read chamber can be washed with warm soapy water and allowed to air dry. Once completely dry, the read chamber may be replaced in the system. See more info about the removable read chamber and other convenient features here.

 

Why are Hygiena's products so much more cost effective?

Hygiena strives to create easier-to-use, more cost-effective products for its clients. By partnering with leading manufacturing companies and using state-of-the-art automated manufacturing processes in house, Hygiena is able to create superior cost-effective products. A perfect example of this is our ATP bioluminescence product line. It incorporates Medical Packaging's patented user-friendly and environmentally-friendly Snap-Valve™ technology, which allows us to create an easy-to-use sample test device that uses less plastic than our competition and has superior performance. Combining this with our unique liquid-stable reagents allows us to have a complete all-in-one sample testing device that has better performance and costs a lot less.

 

How do I contact Hygiena to purchase an ATP solution?

Hygiena products are available worldwide. We have a direct sales staff in the USA, UK and parts of Europe, and Asia. Hygiena also has a network of dedicated distributors and agents across the globe, covering over 80 countries. Hygiena's sales team and distributors have specific skills and experience in providing products in the food processing market, particularly ATP bioluminescence and its applications. To find out more about Hygiena's products or to find a distributor in a particular part of the world, click here.

end faq

 

 

 

Microorganism Tests

MicroSnap Total 

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How fast does MicroSnap Total deliver results?

MicroSnap Total delivers results in 7 hours. This time frame reflects the entire test time, including the enrichment and detection steps.

 

Is it possible to collect my sample ahead of time and run the assay later? For example, I want to collect samples at the end of a shift, and then conduct the test the following day.

Yes. If you are testing for qualitative results (presence/absence), then it is possible to collect sample in advance and delay the assay. To do this, collect the sample according to kit insert instructions and put the entire unactivated enrichment device in a refrigerator (2-8C). When you are ready to begin the test process, remove the enrichment device from the refrigerator and activate the device according to kit insert instructions. Advance sample collection for enumeration testing has not been thoroughly tested by Hygiena laboratories and is not recommended.

 

What happens if I incubate the enrichment device for longer than the 7 hours directed?

If you are looking for presence/absence results, additional incubation will only heighten test sensitivity, so extended incubation is not harmful. However, if you are looking for enumeration results, only incubate the enrichment device for the directed 7 hours.

 

end faq

 

 

MicroSnap Coliform

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How fast does Microsnap Coliform deliver results?

MicroSnap Coliform delivers results in 6-8 hours from sample collection to results.  Enumeration or quantitative results are available in 6 hours. Presence/absence or qualitative results are available in 8 hours. This time frames reflect the entire test time, including the enrichment and detection steps.

 

What happens if I incubate the enrichment device for longer than the time in the directions?

If you are looking for presence/absence results, additional incubation will only heighten test sensitivity, so extended incubation is not harmful. However, if you are looking for enumeration results, only incubate the enrichment device for the directed 6 hours.

 

Is it possible to collect my sample ahead of time and run the assay later? For example, I want to collect samples at the end of a shift, and then conduct the test the following day.

Yes. If you are testing for qualitative results (presence/absence), then it is possible to collect sample in advance and delay the assay. To do this, collect the sample according to kit insert instructions and put the entire unactivated enrichment device in a refrigerator (2-8C). When you are ready to begin the test process, remove the enrichment device from the refrigerator and activate the device according to kit insert instructions. Advance sample collection for enumeration testing has not been thoroughly tested by Hygiena laboratories and is not recommended at this time.

 

end faq

 

 

MicroSnap E. coli

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How fast does MicroSnap E. coli deliver results?

MicroSnap E. coli delivers results in 6-8 hours from sample collection to results.  Enumeration or quantitative results are available in 6 hours. Presence/absence or qualitative results are available in 8 hours. This time frames reflect the entire test time, including the enrichment and detection steps.

 

What happens if I incubate the enrichment device for longer than the time in the directions?

If you are looking for presence/absence results, additional incubation will only heighten test sensitivity, so extended incubation is not harmful. However, if you are looking for enumeration results, only incubate the enrichment device for the directed 6 hours.

 

Is it possible to collect my sample ahead of time and run the assay later? For example, I want to collect samples at the end of a shift, and then conduct the test the following day.

Yes. If you are testing for qualitative results (presence/absence), then it is possible to collect sample in advance and delay the assay. To do this, collect the sample according to kit insert instructions and put the entire unactivated enrichment device in a refrigerator (2-8C). When you are ready to begin the test process, remove the enrichment device from the refrigerator and activate the device according to kit insert instructions. Advance sample collection for enumeration testing has not been thoroughly tested by Hygiena laboratories and is not recommended.

 

end faq

 

 

InSite 

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What strains of Listeria does InSite detect?

InSite detects Listeria monocytogenes, L. innocua, L. ivanovii, and L. welshmeri strains.

 

How do I dispose of InSite?

Autoclave or soak in bleach solution for 1 hour, then dispose of InSite in the trash.

 

I see black spots on the InSite swab bud. Is that positive?

Yellow/Amber media with back spots on the swab bud should be considered as a presumptive negative for Listeria spp.

 

I see very small orange dots occasionally in the media. Is something wrong with the test?

A naturally occurring orange precipitation may be seen in some devices. This is due to excess iron falling out of solution. To alleviate this; the device can be shaken gently to dissolve, either before or after incubation, and used as normal. This will not affect the test result.

 

The media turned brown/black after incubating for longer than 48 hours, is this a presumptive positive?

All tests should be considered negative if the color of the media hasn't changed to brown/black after 48 hours. The media could turn brown/black after 48 hours without the presence of Listeria spp. present. This has to do with the antibiotics in the media being used up and other organisms overwhelming the chemistry. The media after 48 hours will pick up other organisms or fungi that produce the enzyme capable of producing the brown/black color. See the following technical document on catalase testing for test that turn brown/black before 48 hours. InSite Listeria Troubleshooting

 

How do I confirm presumptive positives?

Presumptive positive samples can be confirmed by streaking samples onto commonly used selective Listeria agar plates such as Modified Oxford Agar, Palcam agars or any recognized confirmatory procedure. Typical Listeria colonies on selective agar plates could then be further analyzed by more definitive tests such as microscopy, biochemical tests, etc. Some Enterococcus spp. are capable of giving false positive reactions with the test. All Listeria spp. are catalase positive and Enterococcus spp. are catalase negative, a simple catalase test would distinguish Listeria from Enterococcus spp. See instructions here: InSite Listeria Troubleshooting

 

Are there any organisms that produce a false positive?

Some Enterococcus spp. are capable of giving false positive reactions with the test. All Listeria spp. are catalase positive and Enterococcus spp. are catalase negative, a simple catalase test would distinguish Listeria from Enterococcus spp. See instructions here: InSite Listeria Troubleshooting

  

Is InSite AOAC approved?

InSite is not AOAC approved as a device. However, the broth in the device is AOAC approved.

  

end faq

 

 

Allergen Prevention Tests

AllerSnap

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Do I need to refrigerate AllerSnap?

No. AllerSnap may be stored at room temperatures. The test devices are shipped on ice to compensate for high temperatures in transit. Upon receipt, the swabs may be moved to room temperature storage for the shelf life of the product.

 

What does it mean if the reagent color changes after the time recommended by the kit insert?

Any color change should be disregarded after the recommended time frame. For AllerSnap, any color change after 30 minutes should be ignored.

 

The reagent turned grey. What does this mean?

Any color change within the incubation time  (37°C for 30 minutes or 55°C for 15 minutes) indicates the presence of proteins. A grey color indicates a very small amount of protein was detected. For critical areas or high-risk products, a grey color should prompt a reclean and retest. For low risk areas or products, this should be considered a cautionary result. 

 

What is the difference between ProClean and AllerSnap tests?

AllerSnap is more sensitive than ProClean and can detect to far lower levels of protein. AllerSnap has a 15-30 minute incubation step in the test process.

 

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Surface Residue Tests 

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ProClean 

What does it mean if the reagent color changes after the time recommended by the kit insert?

Any color change should be disregarded after the recommended time frame. For ProClean, any color change after 10 minutes should be ignored.  

Do I need to refrigerate ProClean?

No. ProClean may be stored at room temperatures. The test devices are shipped on ice to compensate for high temperatures in transit. Upon receipt, the swabs may be moved to room temperature storage for the shelf life of the product.

 

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SpotCheck Plus

What does it mean if the reagent color changes after the time recommended by the kit insert?

Any color change should be disregarded after the recommended time frame. For SpotCheck Plus, any color change after 2 minutes should be ignored.

 

Do I need to refrigerate SpotCheck Plus?

Yes. SpotCheck Plus should be refrigerated, but may be stored at room temperature for 4 week shelf life. 

 

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Process Efficiency Tests 

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CrossCheck

How fast does CrossCheck deliver results?

CrossCheck delivers results in 2-5 minutes. You may choose to do a 2 minute quick test or a 5 minute enhanced sensitivity test. The enhanced sensitivity method requires incubation at 37°C.

 

What is the difference between CrossCheck and other rapid tests for Acid Phosphatase?

CrossCheck uses a unique liquid-stable reagent that eliminates the need to reconstitute a lyophilized pellet, giving more accurate results with less variation.  CrossCheck devices are designed for superior efficiency, using less materials in the swab design for maximum sample recovery and consistent results. 

  

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ZymoSnap ALP

Can ZymoSnap ALP be used with all types of milk?

Yes, ZymoSnap ALP may be used with any type of animal's milk. The test can be used for milks, creams, and flavored milk products. 

  

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Sample Collection 

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Do sponge products come with gloves?

All sponge products have the option to come with attached sterile gloves.

 

Can I order sponges dry/without diluent?

Yes. All sponge products are available in a dry format.

 

What diluents are available with sponge products?

All sponge products are available with Buffered Peptone Water, Neutralizing Buffer, Letheen Broth, or dry (no diluent). 

 

What diluents are available with Q-Swab?

Q-Swabs are available with Buffered Peptone Water and Letheen Broth. 

 

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SureTrend Software 

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I have an old version of SureTrend software. How can I get the latest version?

Hygiena can send you a link to download the latest version. Either chat with us via live chat,  or contact tech support by filling out this form. For detailed instructions, follow this PDF guide: How to Upgrade Suretrend 2.02a or Earlier

What OS is recommended for Hygiena's SureTrend Software?

Windows 7 is the preferred operating system for SureTrend software.  Other system reccomendations include:

COMPONENT

REQUIREMENT

Processor

Pentium series processor or higher. x86 (32-bit) and x64 (64-bit) supported

Memory

256 megabyte (MB) RAM or higher

Hard disk

600 megabytes (MB) of free space.

Operating system         

Microsoft Windows XP with Service Pack (SP2), Windows Vista, Windows 7, Windows 8 

Communication

USB or Serial RS232 port

 

Is there a version of SureTrend for Mac?

No, SureTrend was designed for Windows, but you can run SureTrend on a Mac using software like Parallels or other virtualization software. The important requirement for the virtual machine is USB support. Some older versions of Parallels, VMWare, Microsoft Virtual PC do not support USB, so it is important to have a current version. If SureTrend is installed on a terminal server or if the user is remoting to the virtual machine, there may be issues with USB redirection.

 

Does SureTrend support Virtual Desktop or RDP?

SureTrend can run on a Windows Terminal Servier (RDS), Citrix, or other VNC software that will remote the COM port from the server to the desktop. Since SureTrend is running on the server, but the unit is connected to the desktop COM port, communication to the Unit is directed over the network. If your network latency is too high you may get COM port communication failures that require you to repeat the synchronization process with the Unit. Also, the SureTrend feature that automatically detects the COM port will not work and user must know the COM port used on the desktop.

 

Does SureTrend support virtualization such as Windows XP mode or VMWare?

SureTrend will run on virtualized version of Windows supported by SureTrend as long as the virtualization software supports COM ports or USB. Some users are running Windows 7 or 8 on their desktop computer, but spend most of their time in a Windows XP mode session to support other legacy applications in their organization. Having SureTrend run in the Windows XP mode helps the user stay in the Windows XP mode and not have to switch to the native desktop just to run SureTrend.

 

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General Questions 

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How do I place an order?

To order any Hygiena products, contact your regional Hygiena office or qualified distributor in your region.
Americas
International
Asia/Asia Pacific

 

 

How can I get a Certificate of Compliance or Certificate of Analysis for a particular product or lot?

Fill out the Technical Support Request form here and indicate the product and serial or lot number for which you need documentation. Our Customer Service team will promptly reply with the documentation you require.

 

 

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