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BAX® System PCR Assay for Genus Listeria 24E

Listeria species are indicator organisms whose detection can indicate that pathogenic Listeria monocytogenes is present. L. monocytogenes can cause serious illness in persons with heightened susceptibility, especially pregnant women and immunocompromised persons. Traditional culture methods can take four days or more to get results, and many rapid methods require a two-stage, 48-hour enrichment. With this BAX® System 24E assay, however, accurate and reliable results are available the next day with a single-step, 24-hour enrichment.

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PCR Assay Genus Listeria 24E

 


Benefits

  • Single-stage enrichment complete in only 24 hours
  • Automated DNA-based analysis
  • Exceptional sensitivity – Detects 104 cfu/mL
  • Tableted reagents reduce operator error
  • Closed-cap system avoids amplicon contamination in the lab
  • LIMS-compatible electronic data for easy storage, sharing and retrieval
  • Designed for efficient workflow and reliable results
  • PCR processing completes in 3.5 hours
  • Can be run in mixed batches with other BAX® System standard assays
  • Validated on meat, seafood, dairy, vegetable and environmental samples
  • Detects all species of Listeria except some strains of L. grayi
  • 100% exclusivity
  • Next-day results with proprietary 24 LEB Complete media

Specifications

  • Target:   Listeria spp (except L. grayii)
  • Matrices:   Dairy, meat, fish, vegetables, environmentals
  • Enrichment time:   24-28 hours
  • PCR time:   3.5 hours
  • Detection level:   104 cfu/mL, after enrichment
  • Validations:
    • AOAC Research Institute - Performance Tested Method #050903 - validated on bagged spinach, processed cheese, frankfurters, cooked shrimp, stainless steel
    • AFNOR - QUA-18/06-07/08 - all human food products and environmentals. Smoked fish requires use of 24E LEB buffer supplement

Catalog No.

Legacy Order Code Hygiena Product Code Description Quantity
D13608135 KIT2003 BAX® System Listeria spp 24E  96 tests per kit
D14175606 N/A BAX® System Listeria spp 24E Enviro Bundle (Kit & Swipes)  

*Each 2.5 kg tub of 24 LEB Complete enrichment media makes 57.5 liters, sufficient for 640 environmental or 255 food samples.