Helping Hospitals Define Clean

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ATP Cleaning Verification vs. Fluorescent Marking Gel


What is your monitoring system telling you?

More hospitals are switching to ATP Cleaning Verification to ensure the cleanliness of patient environments and critical healthcare surfaces. Explore the benefits of ATP Cleaning Verification over fluorescent monitoring in the published literature and customer case studies below.

Learn why ATP Cleaning Verification is a more valuable tool than fluorescent gel in this short video. 

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Meaningful, measurable results.

EVS and Infection Prevention need quick results to turn beds over faster, verify instrumentation is clean, and collect quantifiable data for meaningful analysis. Blacklight detection of fluorescent gel is an adequate training tool but fails to measure the actual removal of biological matter. ATP cleaning verification tells you what fluorescent gel cannot; how clean a surface actually is with quantifiable results.


Proven in published studies.

Published literature has validated ATP Cleaning Verification as proven method for verifying surface hygiene, improving EVS performance, and advancing overall hospital cleanliness. Studies have also demonstrated ATP Cleaning Verification is more proficient at identifying surface contamination than fluorescent marking systems.

Download a compendium of published studies validating ATP Cleaning Verification here

See what healthcare leaders are saying about ATP Cleaning Verification. 

"Visually, things looked clean - but the luminometer said otherwise. This was an eye-opening experience for all of us - hospital staff and management teams included... It's something we could not live without now." 

"ATP testing has truly helped us become aware of our cleaning techniques."

"ATP is the right way to validate the progress of your cleaning program."

Download the complete whitepaper: ATP Testing: A Proven Method to Measure Cleanliness

 Ready to get started with ATP Cleaning Verification?   Contact Hygiena now.

Compare methods side by side.

Managers need reliable data collection and objective measurement techniques to truly know if a surface is clean.  Refer to the table below to see how the various methods compare across desirable attributes.


ATP Monitoring



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Ease of Use

Can it be used by any level user?

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Does it measure without bias?

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Does the method detect microbiological matter?

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Are results numeric and measurable?

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Can results be categorized as Pass/Fail?

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Does the method minimize time investment?

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Low Cost

Are supplies and other costs affordable?

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Training Tool

Does the tool confirm proper cleaning?

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Management Tool

Is the data collected powerful for managers?

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Are results protected from manipulation?

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Software Analysis

Does the product come with software?

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Grand Total

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Ease of use.

ATP Cleaning Verification is extremely easy to use with a minimal amount of training. To see how easy it is, watch a video demo here. Though blacklight is a relatively easy to use method, other shortcomings, detailed below, make it a poor tool for monitoring environmental cleaning.


According to the CDC and published research, an ideal monitoring tool for evaluating environmental cleaning should be objective, or not subject to any user bias. Blacklight inspection systems only measure the removal of fluorescent gels, so they are not objectively measuring cleanliness of a surface.


Blacklight inspection systems only evaluate if the fluorescent gel was removed from a surface - they cannot measure actual surface contamination. ATP tests detect all organic matter, which includes microbiological material, and deliver those results quickly in 15 seconds. Though ATP testing cannot replace microbiology testing for the identification of pathogens and specific microorganisms, ATP testing can serve as an indicator for potential microbiological matter.


 ATP Cleaning Verification gives a numeric measurement of organic material (ATP) on a surface, measured in relative light units (RLU). The more ATP present on a surface, the higher the RLU measurement. Blacklight inspection cannot quantify the amount of organic material on a surface. Learn more about acceptable RLU scores for hospital locations here.


The SystemSURE Plus displays a Pass, Caution, or Fail symbol on the screen when tests are measured, so results are clear and no interpretation is necessary. Visit this page to see more features on Hygiena’s ATP Cleaning Verification System.


Blacklight inspection requires managers to visit a patient room twice; first, after it has been vacated, before cleaning, to mark locations with fluorescent gel. After the room has been cleaned, management must return to the room a second time to inspect for removal of fluorescent gel marks. Since rooms are continually being turned over, managers are constantly visiting rooms to mark and inspect, taking away valuable time to accomplish other tasks. ATP Cleaning Verification Systems only require one visit to a room, after cleaning, where any surface can be swabbed and results are ready in 15 seconds.

Low cost.

With Hygiena’s ATP Cleaning Verification system, there are zero costs for software licenses, equipment leases, or yearly maintenance. The only costs associated with Hygiena’s ATP Cleaning Verification are the consumable swabs used. An ATP Cleaning Verification system is an affordable way to measure and manage cleaning. To see how much an ATP Cleaning Verification program will cost your hospital, visit the calculator available here, or contact us for a quote

Training tool.

ATP Cleaning Verification is a quick and easy way to show trainees if their cleaning efforts are effective. In 15 seconds, managers can measure ATP residues left behind after cleaning and show trainees an objective, numeric measurement of cleanliness. ATP Cleaning Verification can also be used as a hand washing training tool. Download this document for more information: Monitoring Hand Hygiene Using the Hygiena ATP Cleaning Verification System

Management tool.

The data collected by Hygiena’s ATP Cleaning Verification system provides reports and data that offer powerful decision-making tools for managers. Managers can quickly identify trends, areas for improvement, or staff that may require retraining. ATP test scores can be tracked over time and compared to infection rates. Users typically see that a reduction in RLU scores (a cleaner facility) result in lower infection rates. In a case study at North Tees and Hartlepool hospitals in the United Kingdom, the implementation of ATP Cleaning Verification resulted in a drastic drop in C. diff infection (CDI) rates. Download the case study here: Case Study: North Tees and Hartlepool Hospitals Reduce HAI with Hygiena ATP

Fraud proof.

Unlike blacklight inspection tracking methods, ATP test results cannot be manipulated. Once tests are measured in Hygiena’s ATP Cleaning Verification system, they cannot be edited, overwritten, or deleted. Users cannot know if an ATP test will pass or fail until it is measured. With blacklight systems, cleaning personnel could easily use their own blacklight flashlight to search for spots in a room to clean.

Software analysis.

 ATP Cleaning Verification is the only method with integrated software that allows users to store data, track trends, and analyze results. Learn more about Hygiena’s complimentary SureTrend software here.