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How End-Point Detection Works
In standard assays, BAX® System PCR tablets contain intercalating dye, which binds with double-stranded DNA and emits fluorescent signal in response to light. After the amplification phase is complete, the temperature of the samples is raised to the point where the double-stranded DNA begins to denature. This releases the intercalating dye, which lowers the fluorescent signal. The change in signal can be plotted against temperature to generate a melting curve, which is interpreted by the BAX® System software as positive or negative results.
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