Hygiena presents results of BAX® System PCR analyses of contaminants in foods at International Association for Food Protection

Hygiena, a leader in food safety testing, presented findings from testing the BAX® System PCR method on a variety of important food and environmental matrices. The results were presented at the International Association for Food Protection (IAFP) annual meeting in Louisville, KY.

The studies show that the BAX® System Real-Time PCR method could effectively detect Salmonella in mango, E. coli O157:H7 in beef trim, Listeria monocytogenes in quick-frozen vegetables, and genus Listeria and L. monocytogenes in a variety of foods and environmental samples.

“We are encouraged by these studies that show the effectiveness of the BAX® Real-Time PCR system compared to reference methods in several high-risk food samples,” said Martin Easter, Chief Scientific Officer of Hygiena. “We believe using these real-time PCR assays and instruments will provide results more rapidly, providing information vital to maintaining a safe food supply.”

The abstracts can be found on the IAFP website, or by clicking on the links below:

Fresh cut mango—Using the BAX® System Real-Time PCR assay for Salmonella to detect Salmonella in 375 g or 25 g of fresh cut mango, demonstrated no significant statistical difference between the real-time PCR and the reference method.

Beef trim and surface sampling—Researchers evaluated the performance of the BAX® System PCR Real-Time PCR assay for E. coli O157:H7 to detect E. coli O157:H7 from individual and pooled samples of MicroTally sampling swabs from beef trim, in comparison to the USDA-FSIS reference culture method. The study showed that beef producers can reliably use the BAX® System to detect E. coli O157:H7 contamination from surface swabs for beef trimmings.

Quick frozen vegetables--Since L. monocytogenes can survive freezing temperatures, there are increased concerns in frozen products. The BAX® real-time PCR assay for L. monocytogenes was evaluated in frozen vegetables to minimize the risk that contaminated product will reach consumers. The BAX® real-time L. monocytogenes PCR assay returned positive results for samples of broccoli, corn, carrots, and peas. All presumptive results were identical to culture, with no difference in detection from the reference method.

Genus Listeria and L. monocytogenes—Two BAX® real-time PCR assays for screening genus Listeria species and L. monocytogenes were evaluated in five food categories and one environmental category. The assays exhibited increased assay sensitivity and faster sample preparation and cycling times than endpoint PCR detection. These new assays allow for rapid time-to-results for testing food/environmental samples, while preserving ease, accuracy and dependability.

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