News from Hygiena

Improved assay reduces time to product release by detecting bacteria faster

 

CAMARILLO, Calif. (23 October 2020)— Hygiena, a leader in food safety testing, announced the NEW BAX® System Real-Time PCR Assay for E. coli O157:H7 Exact, a test that can specifically identify Escherichia coli O157:H7 bacteria (and not similar serotypes) in less time than other tests.

The BAX® technology is based on the polymerase chain reaction (PCR) method of amplifying minute amounts of genetic material in a sample for more accurate measurement. The new BAX® System Real-Time Assay for E. coli O157:H7 Exact is based on a single genetic target in E. coli O157:H7, providing a definitive result for immediate action and minimizes the need for additional confirmatory testing.

Improved chemistry in the new assay allows detection as low as 104 colony forming units (CFU/mL) in an enriched sample, opening the door to reduced enrichment times and faster time to results for quicker product release.

The assay can be run simultaneously with the BAX® System Real-Time PCR assay suite for STEC, including the BAX® System Real-Time STEC screening assay, and BAX® System Real-Time PCR assays for STEC Panel 1 and STEC Panel 2.

Hygiena’s validation testing of the BAX® System Real-Time PCR Assay for E. coli O157:H7 Exact in raw ground beef, beef trim, leafy greens and raw milk showed no significant differences in results compared to the reference conventional culture confirmation method. In addition, the test successfully identified 239 strains of E. coli O157:H7 and did not identify 300 non-O157:H7 strains or 175 non-E. coli bacteria.

“We are so excited to introduce this new assay to the BAX® System! This assay will dramatically improve the accuracy and speed of testing for E. coli O157:H7, providing the industry a needed tool for product release,” said April Englishbey, PhD., product manager at Hygiena. “We are confident that this assay’s ability for improved results in a variety of tough matrices will help improve the safety of food supply chains.”

ABOUT HYGIENA

Hygiena delivers rapid microbial detection, monitoring, and identification solutions to a wide range of industries, including food and beverage, healthcare, hospitality, pharmaceuticals, and personal care. Utilizing advanced technologies and patented designs, Hygiena provides industry-leading ATP monitoring systems, PCR-based pathogen detection and characterization systems, allergen tests, mycotoxin tests, environmental collection devices, and more. Hygiena is committed to the mission of providing customers with high-quality innovative technologies that are easy-to-use and reliable, backed by excellent customer service and support. Headquartered in Camarillo, California with offices in Wilmington, Delaware, Mexico, Spain, Canada, the United Kingdom and China, and over 80 distributors in more than 100 countries worldwide, Hygiena products span the globe. www.hygiena.com.

Hygiena Adds Specific Walnut Test to the AlerTox Range

CAMARILLO, Calif. (13 October 2020)— Hygiena, a leader in food safety testing, is excited to launch a new test to increase its rapid test line of allergens, Alertox® Sticks Walnut. This line of rapid test for allergen detection is designed for the food processing industry to support allergen control programs and labelling requirements.

This lateral flow strip has a limit of detection of 2ppm of walnut protein in food samples.  It is also validated of surfaces for cleaning verification.  Using monoclonal antibodies to detect allergenic protein in walnut (Juglans regia), the test does not cross-react with the closely related tree nut pecan (Carya illinoinensis), or other allergens

This product represents a cost effective and rapid solution as the procedure only takes a few minutes and there is no equipment required as all additional elements are included in the 10 test pack. The test can be used for incoming ingredients, finished products or surfaces. It is validated in a wide range of products such as protein bars, bread, dairy products, bakery products, cheese and stainless steel.

Hygiena’s Alertox line of rapid allergen tests includes milk (casein and beta-lactoglobulin separately or combined), egg, fish, crustacean, soy, mustard seed, almond and hazelnut (both for food analysis and surface testing). –

Contact Hygiena for further information and learn more about the range of allergen tests.

ABOUT HYGIENA

Hygiena delivers rapid microbial detection, monitoring, and identification solutions to a wide range of industries, including food and beverage, healthcare, hospitality, pharmaceuticals, and personal care. Utilizing advanced technologies and patented designs, Hygiena provides industry-leading ATP monitoring systems, PCR-based pathogen detection and characterization systems, allergen tests, mycotoxin tests, environmental collection devices, and more. Hygiena is committed to the mission of providing customers with high-quality innovative technologies that are easy-to-use and reliable, backed by excellent customer service and support. Headquartered in Camarillo, California with offices in Wilmington, Delaware, Mexico, Spain, Canada, the United Kingdom and China, and over 80 distributors in more than 100 countries worldwide, Hygiena products span the globe. www.hygiena.com.

Hygiena Releases GlutenTox ELISA Rapid G12, A More Sensitive, Faster Gluten Test

Improved test provides significant advance in determining gluten in food

Hygiena, a leader in food safety testing, announced the release of GlutenTox ELISA Rapid G12, an improved test for the most immunotoxic peptide in gluten. The test provides more accurate results in less time than either previous versions or competitors’ ELISA tests for gluten.

GlutenTox ELISA Rapid G12 uses the G12 antibody to identify in food samples the 33-mer (part) peptide segment of the gliadin protein, which is part of the gluten protein complex. This “33-mer” peptide is the most immunotoxic part of the gluten protein and induces celiac disease in susceptible people. The 33-mer peptide also shows high resistance to complete digestion by human digestive enzymes.  Focusing on this peptide fragment provides more objective information to help people with celiac disease and gluten intolerance to avoid foods that contain gluten.

GlutenTox ELISA Rapid G12 provides fast, convenient and accurate measurement of gluten down to 0.6 ppm (0.3 ppm of gliadin).

GlutenTox ELISA Rapid G12 is based on the enzyme-linked immunoassay (ELISA) test, a proven laboratory workhorse for more than 40 years. This test first uses the G12 antibody that is fixed to a testing surface (the “capture” antibody). This antibody binds specifically to certain peptides (in this case, the 33-mer). A second antibody, the “detection antibody,” is then introduced, labeled with an enzyme so it and its accompanying target protein can be measured. The “capture” antibody determines what proteins the “detection” antibody can pinpoint. 

In addition, standards provided with the kit will be ready-to-use for the first time. This innovation will reduce operator errors, as well as the time needed to prepare assays. The new kit is also more stable during temperature changes, and delivers positive results even in highly contaminated samples (a limitation of other gluten tests). Finally, GlutenTox ELISA Rapid G12 tests have shorter incubation times, allowing for an accurate-analytic measurement of gluten in 90 minutes.

“With additional benefits like faster time to results, less susceptibility to temperature and nontoxic reagents, we are confident this new test will mark a significant advance in the ability to detect gluten proteins that are so harmful to those sensitive to even low levels of gluten.,” said Charles McGuill, Product Manager at Hygiena. 

Hygiena™ EnSURE™ Touch used in School Sanitation Program - KOMO-TV

  

PeachBelt sanitizer

PeachBelt Conference uses EnSURE™ Touch to protect student athletes

The PeachBelt Conference, an NCAA Division II association of collegiate teams in four southern states, is preparing for a safe return of student athletes to its member schools. Part of those preparations include rigorous cleaning and disinfection of facilities, and using EnSURE™ Touch ATP monitoring to verify their cleaning efforts.

The conference consists of 12 schools, in Florida, Georgia, North Carolina and South Carolina. Sports include football, basketball, soccer, tennis, baseball, golf, track and field, and others. Men's and women's teams are represented in the conference.

PBC commissioner David Brunk said, "We wanted to do something tangible to support our schools and create the safest environment for our student-athletes to return. After speaking with some of our corporate partners who are experts in these areas, we knew that the best thing we could do to help is provide these misting machines to sanitize surfaces as well as testing machines and swabs to ensure that the levels of organic material were below CDC thresholds."

The conference purchased misting sanitizer that will adhere to any surface, as well as a Hygiena ATP luminometer and UltraSnap testing swabs for each member school.

Learn more about the PeachBelt Conference and its cleaning efforts here.

Actero™ Elite Enrichment Media for the BAX® System reduces sample handling, improves throughput and time to results

 

(31 May 2018) Hygiena, a Warburg Pincus portfolio company that specializes in rapid food safety and environmental sanitation testing, announced that it has begun selling Actero™ Elite Enrichment Media, validated for use with the BAX® System real-time PCR assays for Listeria and Salmonella.
The two Actero™ Elite Enrichment Media formulations, produced by FoodChek Systems, Inc., are known in the industry for superior quality, single-step enrichment allowing for reduced sample handling, reduced enrichment media volumes, and faster time to results.
“We are delighted to be able to provide Actero™ Elite Enrichment Media to our BAX® System customers, which enables them to improve their ability to detect pathogens in food or environmental samples,” said Shannon Bullard, global product manager at Hygiena. “This partnership provides customers with industry leading technology providing accurate and efficient pathogen detection that is easy to use resulting in faster product release.”
William Hogan, FoodChek President & CEO, said, “We are very pleased with the opportunity of working with Hygiena and its outstanding food safety testing expertise. Hygiene’s competencies, reputation of food safety excellence and global reach make them an ideal partner for FoodChek in bringing our Actero Elite Enrichment Media to the food safety testing market.”
The Actero™ Elite Enrichment Media is formulated to suppress the growth of non-target organisms while promoting the growth of target organisms. Together, Actero™ Elite Enrichment Media and the BAX® System can provide environmental detection results in 22 hours for Listeria and 16 hours for Salmonella. The products are available in powder and liquid MediaBox™ formats. Other benefits of each media formulation are:
• For Listeria species assays, time to results can occur between 18-26 hours depending on sample source, and they feature a single-step enrichment.
• For Salmonella species assays, time to results can occur between 14-26 hours depending on sample source, and they feature a single-step enrichment.
Hygiena’s BAX® System PCR Assays have been certified as a Performance Tested Method by the AOAC Research Institute (AOAC-RI), and are also approved by Health Canada, the country’s national health agency, for use in food and environmental surface samples.

ABOUT FOODCHEK
The FoodChek Systems Inc. mission is to be the leading global developer and provider of proprietary rapid and accurate food safety tests for the detection of foodborne and environmental pathogens and other microorganisms throughout the human and pet food production chains. The company's expertise is in the development and commercialization of proprietary rapid, accurate and cost-effective food safety tests focused on the detection of Listeria spp., Listeria monocytogenes, and Salmonella spp. under the Actero™ Elite Enrichment Media brand of products. FoodChek is headquartered in Calgary, Alberta, Canada.

 

Bus Cleaning

American Public Transit Association cleaning/sanitation guide includes Hygiena ATP tests

The American Public Transit Association (APTA), the organization representing all transportation agencies in the US, published a guide to cleaning and disinfecting buses, trains, and other facilities in response to COVID-19. The APTA Cleaning and Disinfecting Guidance details steps that should be taken to ensure cleanliness and includes the use of ATP monitoring, and illustrates the use of Hygiena's ATP luminometers and detection systems in public transit. 

Learn more and read the paper here. 

Test in milk products has broadest, most sensitive detection in industry

Hygiena’s Helica Biosystems has introduced a new test for aflatoxin M1, a carcinogenic chemical found in milk products. Studies found the enzyme-linked immunoassay (ELISA), Aflatoxin M1 Ultra ELISA Quantitative, is more sensitive than competitors’ tests for detecting the toxin.

Aflatoxin M1 is found in the milk of cows that eat Aflatoxin B1-contaminated feed. Aflatoxin B1 is one of the world’s most carcinogenic compounds. It is produced by molds including Aspergillis flavus and A. parasiticus, commonly found in feeds. While not as toxic as its parent compound Aflatoxin B1, Aflatoxin M1 can still cause cancers, immune system disorders and severe toxic reactions. Dairy processors need fast, accurate tests that detect low levels of Aflatoxin M1 in their milk, milk powder and yogurt products.

In the United States, the highest concentration of Aflatoxin M1 allowed in products is 500 parts per trillion (ppt). In the European Union, that limit is 50 ppt. In addition, ISO 14675 guidelines require a minimum quantification of 10 ppt, less than 20 percent cross-reactivity to the related Aflatoxin M2, and a standard range of 5-50 ppt.

The new Aflatoxin M1 Ultra ELISA Quantitative has a broad detection range of 5 ppt to 500 ppt. The ELISA assay takes only 1 hour, 10 minutes to generate results. This year, an independent laboratory verified the performance of Aflatoxin M1 Ultra ELISA Quantitative with a competing kit, validating its 5-500 ppt detection in milk samples, with high sample recovery, and high testing precision and accuracy. All Hygiena assay outperformed competing kit.

Aflatoxin M1 Ultra ELISA also shows less than 0.1% cross-reactivity to other aflatoxin types (M2, B1, B2, G1 and G2) that could cause false-positive results in the presence of these other aflatoxin types. These types can exist in barley, walnut, oats and other grains and nuts often added to dairy products.

“Hygiena’s Helica brand of mycotoxin tests has been supplying dairy markets worldwide for 15 years with effective assays,” said Charles McGuill, product manager at Hygiena. “The new high sensitivity aflatoxin M1 assay ELISA kit meets all regulatory requirements and effectively determines the presence of aflatoxin M1.”

2020 Ensure Touch (Covid 19) ABC-10 

 

COVID 19 image

How ATP Cleaning Verification can help!

In times like these, knowledge is power.

Hygiena™ has been closely monitoring the Coronavirus (COVID-19) situation and the implications it has for our employees, customers and communities. During this time we have received a multitude of questions regarding our solutions as they pertain to the situation.

Due to this, we wanted to take this time to explain how our ATP Cleaning Verification products work and are suggested to be used in this troubling time.

 

How Hygiena ATP Cleaning Verification Products Work:

  • Hygiena's cleaning verification products can tell you in as little as 10 seconds if a surface has been cleaned properly using ATP.
  • ATP is an energy molecule found in all plant, animal and microbial cells. It fuels metabolic processes such as cellular reproduction, muscle contraction, plant photosynthesis, respiration in fungi, and fermentation in yeast. All organic matter (living or once-living) contains ATP, including food, bacteria, mold and other microorganisms.
  • Hygiena™ ATP testing devices contain a natural enzyme found in fireflies. This enzyme, called luciferase, produces a simple bioluminescence (lightproducing) reaction when it comes into contact with ATP. Using bioluminescence technology, our ATP Meters can measure extremely low levels of ATP collected with testing devices. Measuring the amount of bioluminescence from an ATP reaction provides an excellent indication of surface cleanliness because the quantity of light generated by the reaction is directly proportional to the amount of ATP present in the sample.

 

How Hygiena ATP Cleaning Verification Products can be used:

 

On the other hand, we wanted to clarify a couple points about our products' capabilities.

 

Relevant Hygiena Product Information:

  • ATP systems do not detect viruses directly. However, COVID-19 infected material that contains biological residues will be detected by an ATP system.
  • Hygiena does not produce a virus test
  • Performing ATP CleaningVerification does not guarantee removal of viruses on surfaces
  • ATP Cleaning Verification should be used in conjunction with CDC recommendations and guidelines

If you have questions about our products or are interested in using them in your facility, please click the button below to contact a Hygiena representative in your area.

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