News from Hygiena

Actero™ Elite Enrichment Media for the BAX® System reduces sample handling, improves throughput and time to results


(31 May 2018) Hygiena, a Warburg Pincus portfolio company that specializes in rapid food safety and environmental sanitation testing, announced that it has begun selling Actero™ Elite Enrichment Media, validated for use with the BAX® System real-time PCR assays for Listeria and Salmonella.
The two Actero™ Elite Enrichment Media formulations, produced by FoodChek Systems, Inc., are known in the industry for superior quality, single-step enrichment allowing for reduced sample handling, reduced enrichment media volumes, and faster time to results.
“We are delighted to be able to provide Actero™ Elite Enrichment Media to our BAX® System customers, which enables them to improve their ability to detect pathogens in food or environmental samples,” said Shannon Bullard, global product manager at Hygiena. “This partnership provides customers with industry leading technology providing accurate and efficient pathogen detection that is easy to use resulting in faster product release.”
William Hogan, FoodChek President & CEO, said, “We are very pleased with the opportunity of working with Hygiena and its outstanding food safety testing expertise. Hygiene’s competencies, reputation of food safety excellence and global reach make them an ideal partner for FoodChek in bringing our Actero Elite Enrichment Media to the food safety testing market.”
The Actero™ Elite Enrichment Media is formulated to suppress the growth of non-target organisms while promoting the growth of target organisms. Together, Actero™ Elite Enrichment Media and the BAX® System can provide environmental detection results in 22 hours for Listeria and 16 hours for Salmonella. The products are available in powder and liquid MediaBox™ formats. Other benefits of each media formulation are:
• For Listeria species assays, time to results can occur between 18-26 hours depending on sample source, and they feature a single-step enrichment.
• For Salmonella species assays, time to results can occur between 14-26 hours depending on sample source, and they feature a single-step enrichment.
Hygiena’s BAX® System PCR Assays have been certified as a Performance Tested Method by the AOAC Research Institute (AOAC-RI), and are also approved by Health Canada, the country’s national health agency, for use in food and environmental surface samples.

The FoodChek Systems Inc. mission is to be the leading global developer and provider of proprietary rapid and accurate food safety tests for the detection of foodborne and environmental pathogens and other microorganisms throughout the human and pet food production chains. The company's expertise is in the development and commercialization of proprietary rapid, accurate and cost-effective food safety tests focused on the detection of Listeria spp., Listeria monocytogenes, and Salmonella spp. under the Actero™ Elite Enrichment Media brand of products. FoodChek is headquartered in Calgary, Alberta, Canada.


Bus Cleaning

American Public Transit Association cleaning/sanitation guide includes Hygiena ATP tests

The American Public Transit Association (APTA), the organization representing all transportation agencies in the US, published a guide to cleaning and disinfecting buses, trains, and other facilities in response to COVID-19. The APTA Cleaning and Disinfecting Guidance details steps that should be taken to ensure cleanliness and includes the use of ATP monitoring, and illustrates the use of Hygiena's ATP luminometers and detection systems in public transit. 

Learn more and read the paper here. 

PeachBelt sanitizer

PeachBelt Conference uses EnSURE™ Touch to protect student athletes

The PeachBelt Conference, an NCAA Division II association of collegiate teams in four southern states, is preparing for a safe return of student athletes to its member schools. Part of those preparations include rigorous cleaning and disinfection of facilities, and using EnSURE™ Touch ATP monitoring to verify their cleaning efforts.

The conference consists of 12 schools, in Florida, Georgia, North Carolina and South Carolina. Sports include football, basketball, soccer, tennis, baseball, golf, track and field, and others. Men's and women's teams are represented in the conference.

PBC commissioner David Brunk said, "We wanted to do something tangible to support our schools and create the safest environment for our student-athletes to return. After speaking with some of our corporate partners who are experts in these areas, we knew that the best thing we could do to help is provide these misting machines to sanitize surfaces as well as testing machines and swabs to ensure that the levels of organic material were below CDC thresholds."

The conference purchased misting sanitizer that will adhere to any surface, as well as a Hygiena ATP luminometer and UltraSnap testing swabs for each member school.

Learn more about the PeachBelt Conference and its cleaning efforts here.

Test in milk products has broadest, most sensitive detection in industry

Hygiena’s Helica Biosystems has introduced a new test for aflatoxin M1, a carcinogenic chemical found in milk products. Studies found the enzyme-linked immunoassay (ELISA), Aflatoxin M1 Ultra ELISA Quantitative, is more sensitive than competitors’ tests for detecting the toxin.

Aflatoxin M1 is found in the milk of cows that eat Aflatoxin B1-contaminated feed. Aflatoxin B1 is one of the world’s most carcinogenic compounds. It is produced by molds including Aspergillis flavus and A. parasiticus, commonly found in feeds. While not as toxic as its parent compound Aflatoxin B1, Aflatoxin M1 can still cause cancers, immune system disorders and severe toxic reactions. Dairy processors need fast, accurate tests that detect low levels of Aflatoxin M1 in their milk, milk powder and yogurt products.

In the United States, the highest concentration of Aflatoxin M1 allowed in products is 500 parts per trillion (ppt). In the European Union, that limit is 50 ppt. In addition, ISO 14675 guidelines require a minimum quantification of 10 ppt, less than 20 percent cross-reactivity to the related Aflatoxin M2, and a standard range of 5-50 ppt.

The new Aflatoxin M1 Ultra ELISA Quantitative has a broad detection range of 5 ppt to 500 ppt. The ELISA assay takes only 1 hour, 10 minutes to generate results. This year, an independent laboratory verified the performance of Aflatoxin M1 Ultra ELISA Quantitative with a competing kit, validating its 5-500 ppt detection in milk samples, with high sample recovery, and high testing precision and accuracy. All Hygiena assay outperformed competing kit.

Aflatoxin M1 Ultra ELISA also shows less than 0.1% cross-reactivity to other aflatoxin types (M2, B1, B2, G1 and G2) that could cause false-positive results in the presence of these other aflatoxin types. These types can exist in barley, walnut, oats and other grains and nuts often added to dairy products.

“Hygiena’s Helica brand of mycotoxin tests has been supplying dairy markets worldwide for 15 years with effective assays,” said Charles McGuill, product manager at Hygiena. “The new high sensitivity aflatoxin M1 assay ELISA kit meets all regulatory requirements and effectively determines the presence of aflatoxin M1.”

2020 Ensure Touch (Covid 19) ABC-10 


COVID 19 image

How ATP Cleaning Verification can help!

In times like these, knowledge is power.

Hygiena™ has been closely monitoring the Coronavirus (COVID-19) situation and the implications it has for our employees, customers and communities. During this time we have received a multitude of questions regarding our solutions as they pertain to the situation.

Due to this, we wanted to take this time to explain how our ATP Cleaning Verification products work and are suggested to be used in this troubling time.


How Hygiena ATP Cleaning Verification Products Work:

  • Hygiena's cleaning verification products can tell you in as little as 10 seconds if a surface has been cleaned properly using ATP.
  • ATP is an energy molecule found in all plant, animal and microbial cells. It fuels metabolic processes such as cellular reproduction, muscle contraction, plant photosynthesis, respiration in fungi, and fermentation in yeast. All organic matter (living or once-living) contains ATP, including food, bacteria, mold and other microorganisms.
  • Hygiena™ ATP testing devices contain a natural enzyme found in fireflies. This enzyme, called luciferase, produces a simple bioluminescence (lightproducing) reaction when it comes into contact with ATP. Using bioluminescence technology, our ATP Meters can measure extremely low levels of ATP collected with testing devices. Measuring the amount of bioluminescence from an ATP reaction provides an excellent indication of surface cleanliness because the quantity of light generated by the reaction is directly proportional to the amount of ATP present in the sample.


How Hygiena ATP Cleaning Verification Products can be used:


On the other hand, we wanted to clarify a couple points about our products' capabilities.


Relevant Hygiena Product Information:

  • ATP systems do not detect viruses directly. However, COVID-19 infected material that contains biological residues will be detected by an ATP system.
  • Hygiena does not produce a virus test
  • Performing ATP CleaningVerification does not guarantee removal of viruses on surfaces
  • ATP Cleaning Verification should be used in conjunction with CDC recommendations and guidelines

If you have questions about our products or are interested in using them in your facility, please click the button below to contact a Hygiena representative in your area.

Contact Us 



Jan San Header


What does clean really mean?

Implementing ATP Cleaning Verification in Janitorial and Sanitation Processes 

Hygiena™ ATP Cleaning Verification Systems help organizations achieve optimal standardized cleaning levels. These systems use bioluminescence technology to identify and measure adenosine triphosphate, commonly known as ATP. This measurement helps organizations objectively determine from a microbiological level if a surface has been cleaned properly and is safe for use, or if it requires a corrective action such as re-cleaning. 

- What is ATP? ATP is an energy molecule found in all living cells that allows cellular metabolism to take place. All organic matter contains ATP. In healthcare facilities, organic matter such as bodily fluids, blood, and bacteria left on surfaces can become a point of cross contamination between patients and staff, which can lead to infections if not properly cleaned. Therefore, the detection of ATP on a surface after cleaning is an indication of cleaning efficacy. 

- What is bioluminescence? Hygiena ATP tests contain an enzyme called luciferase which produces a bioluminescence (light-producing) reaction when it comes into contact with ATP. The light emitted from the reaction is measured and quantified in a Hygiena luminometer. The unit of measurement for the emitted light is Relative Light Units (RLU). 

- Higher Contamination = Higher RLU - The quantity of light generated by the bioluminescence reaction is directly proportional to the amount of ATP present in the sample. The reaction is immediate, allowing results to be processed in real-time. Results are expressed numerically on the screen in RLU. 

- How are test results tracked? – Test results can be tracked on your luminometer and software by location, plan, and user. This information allows users to clearly associate results with the specific location, or group of locations tested, and who performed the tests. 


Download the Full Quick Guide Here


As schools, governments, and private businesses alike adopt US Centers for Disease Control guidelines on cleaning and disinfection as a defense against the SARS-Cov-2 coronavirus, many are turning to Hygiena's ATP monitoring technology to help verify cleaning efforts. Studies have shown that certain disinfectants can disable the virus on surfaces. While ATP monitoring can't detect viruses, and scientists still don't know how infective SARS-Cov-2 is from a surface, it's still helpful to at least reduce overall contamination risk from microbes. 

Here, the Richland, Washington, school district reports on using Hygiena's EnSURE Monitoring system in its facilities. Like all Washington state public schools, the district is now closed until April 24, but it has been conducting enhanced sanitation and monitoring. 


Coronavirus (COVID-19) Update

Hygiena has been closely monitoring the Coronavirus (COVID-19) situation and the implications it has for our employees, customers and communities. We will provide ongoing updates below.

Read our full communication.

Date & Time Update
3/16/2020 | 11:15 AM PST Supply chain, manufacturing production and distribution channels are fully operational.

The RiboPrinter has been used by food safety professionals and in healthcare settings to characterize bacteria down to the strain level, but a recent Czech study has extended its capabilities to discovering new species of Staphyloccus in Antarctica. The RiboPrinter helped determine two new species, and also helped scientists find new genes that controlled resistance to antibiotics. The study underscores the reliability and versatility of the RiboPrinter system and illustrates usefulness in more traditional food safety and healthcare settings. You can read about the research in our case study here