Other Home → Allergen Prevention Tests → AlerTox® ELISA Allergens
Highly specific & sensitive allergen detection
AlerTox® ELISA is a quantitative immunosorbent assay designed for the quantitative determination of allergens in raw materials and final products. The ELISA Sandwich is a technique usually used for the analysis of substances found at very low concentrations. This combined with the high specificity and sensitivity of the antibodies used in these tests allow this method to precisely quantify allergens in all types of food and drinks.
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How it works
All AlerTox ELISA tests work on the principle of a quantitative sandwich ELISA. An antibody directed against the target antigen (protein) is bound on the surface of a microtiter plate. Antigen-containing samples or standards are given into the wells of the microtiter plate. After 20 minutes incubation at room temperature (15-25º C/59-77º F), the wells are washed with diluted washing solution to remove unbound material.
A peroxidase-conjugated second antibody directed against the same antigen is given into the wells and after 20 minutes of incubation, the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a Stop Solution, and the color turns yellow. The yellow color is measured photometrically at 450 nm. The concentration of the antigen is directly proportional to the color intensity of the test sample.
Limits of Detection & Quantification:
Round out your allergen prevention program.
For everyday protein residue detection, try AllerSnap. AllerSnap is a quick color-changing swab test that detects a wide range of proteins. Verifying effective protein removal between changeovers is a quick way to ensure sanitation protocols are effectively removing potential allergens on surfaces.
Learn more about AllerSnap!
Benefits
- ELISA Sandwich (96 well) detection
- Highly specific & sensitive
- Easy-to-use
- Large range of measurable allergens
- 12-month shelf life from date of production* (shelf-life is printed on the box)
Specifications
Kit | LOD | LOQ |
AlerTox ELISA Coconut | 0.4 ppm | 2 pp |
AlerTox ELISA Lupine | 0.2 ppm | 2 ppm |
AlerTox ELISA Sesame | 0.2 ppm | 2 ppm |
AlerTox ELISA Mustard | 1.5 ppm | 2 ppm |
AlerTox ELISA Macadamia | 0.1 ppm | 1 ppm |
AlerTox ELISA Cashew | 0.2 ppm | 2 ppm |
AlerTox ELISA Soy (STI) | 16 ppb | 50 ppb |
AlerTox ELISA Walnut | 0.6 ppm | 2 ppm |
AlerTox ELISA Peanut | 0.3 ppm | 1 ppm |
AlerTox ELISA Hazelnut | 0.3 ppm | 1 ppm |
AlerTox ELISA Almond | 0.2 ppm | 0.5 ppm |
AlerTox ELISA Pistachio | 0.13 ppm | 1 ppm |
AlerTox ELISA Egg | 0.05 ppm | 0.4 ppm |
AlerTox ELISA Lysozyme | 2 ppb | 25 ppb |
AlerTox ELISA Ovalbumin | 5 ppb | 25 ppb |
AlerTox ELISA Casein | 0.05 ppm | 0.20 ppn |
AlerTox ELISA Milk | 0.05 ppm | 0.5 ppm |
AlerTox ELISA BLG | 1.5 ppb | 10 ppb |
AlerTox ELISA Crustacean | 1 ppb | 20 ppb |
AlerTox ELISA Fish | 1.4 ppm | 4 ppm |
Catalog No.
Catalog No. | Description | Quantity |
KIT3056 | AlerTox ELISA Coconut | 1 Kit |
KIT3057 | AlerTox ELISA Lupine | 1 Kit |
KIT3051 | AlerTox ELISA Sesame | 1 Kit |
KIT3058 | AlerTox ELISA Mustard (white, black, brown) | 1 Kit |
KIT3055 | AlerTox ELISA Macadamia | 1 Kit |
KIT3053 | AlerTox ELISA Cashew | 1 Kit |
KIT3047 | AlerTox ELISA Soy (STI=Soy Trypsin Inhibitor) | 1 Kit |
KIT3052 | AlerTox ELISA Walnut | 1 Kit |
KIT3048 | AlerTox ELISA Peanut | 1 Kit |
KIT3053 | AlerTox ELISA Hazelnut | 1 Kit |
KIT3049 | AlerTox ELISA Almond | 1 Kit |
KIT3054 | AlerTox ELISA Pistachio | 1 Kit |
KIT3046 | AlerTox ELISA Egg (Egg white proteins) | 1 Kit |
KIT3044 | AlerTox ELISA Lysozyme | 1 Kit |
KIT3045 | AlerTox ELISA Ovalbumin | 1 Kit |
KIT3043 | AlerTox ELISA Casein | 1 Kit |
KIT3041 | AlerTox ELISA Milk (Casein & BLG) | 1 Kit |
KIT3042 | AlerTox ELISA BLG | 1 Kit |
KIT3059 | AlerTox ELISA Crustacean | 1 Kit |
KIT3060 | AlerTox ELISA Fish | 1 Kit |
Resources
Brochure
Instructions
Safety Data Sheet (SDS)
- AlerTox ELISA English US | UK
- AlerTox ELISA Spanish Spain | Mexico
- AlerTox ELISA Italian
- AlerTox ELISA Chinese
Technical Documents
Validation Reports
- Validation Report AlerTox ELISA BLG
- Validation Report AlerTox ELISA Almond
- Validation Report AlerTox ELISA Casein
- Validation Report AlerTox ELISA Cashew
- Validation Report AlerTox ELISA Coconut
- Validation Report AlerTox ELISA Crustacean
- Validation Report AlerTox ELISA Egg
- Validation Report AlerTox ELISA Fish
- Validation Report AlerTox ELISA Hazelnut
- Validation Report AlerTox ELISA Lupine
- Validation Report AlerTox ELISA Lysozyme
- Validation Report AlerTox ELISA Macadamia
- Validation Report AlerTox ELISA Milk
- Validation Report AlerTox ELISA Mustard
- Validation Report AlerTox ELISA Ovalbumin
- Validation Report AlerTox ELISA Peanut
- Validation Report AlerTox ELISA Pistachio
- Validation Report AlerTox ELISA Sesame
- Validation Report AlerTox ELISA Soy
- Validation Report AlerTox ELISA Walnut
Calculation Sheets
- AlerTox ELISA Milk Calculation Sheet
- AlerTox ELISA BLG Calculation Sheet
- AlerTox ELISA Casein Calculation Sheet
- AlerTox ELISA Lysozyme Calculation Sheet
- AlerTox ELISA Ovalbumin Calculation Sheet
- AlerTox ELISA Egg Calculation Sheet
- AlerTox ELISA Soy Calculation Sheet
- AlerTox ELISA Peanut Calculation Sheet
- AlerTox ELISA Almond Calculation Sheet
- AlerTox ELISA Hazelnut Calculation Sheet
- AlerTox ELISA Sesame Calculation Sheet
- AlerTox ELISA Walnut Calculation Sheet
- AlerTox ELISA Pistachio Calculation Sheet
- AlerTox ELISA Coconut Calculation Sheet
- AlerTox ELISA Lupine Calculation Sheet
- AlerTox ELISA Mustard Calculation Sheet
- AlerTox ELISA Crustacean Calculation Sheet
- AlerTox ELISA Fish Calculation Sheet
- AlerTox ELISA Cashew Calculation Sheet
Case Studies
White Papers