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How Real-Time Detection Works

 

how realtime worksThe PCR tablets in BAX® System real-time assays also contain mulitiple, target-specific dye-labeled probes for fluorescent detection. Probes are short DNA sequences with quencher dye at one end that greatly reduces signal from the fluorescent dye at the opposite end. Probes bind to a specific area within the targeted DNA fragment. During PCR the fluorophor separates from the quencher, allowing for increased fluorescent signal. The BAX® System instrument uses dye-specific filters to measure fluorescent signal at the end of each cycle, plotting any amplification and displaying clear positive or negative results at the end of the 50-75 minute program.

 

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