Food & Beverage Home → PCR Pathogen Tests → BAX® System → BAX® System Assays → Shigella
BAX® System Real-time PCR Assay for Shigella
Shigella naturally shares large portions of its' genetic sequence with many other pathogenic bacteria and is very easy to cross-contaminate with very lose dose response required. This cross-contamination primarily occurs from poor hygiene practices during food production and handling in retail environments. Utilizing the Real-Time PCR assay for Shigella can quickly eliminate negative samples and reduce presumptive positives seen in conventional methods due to the specificity and inclusivity of serotypes. Common implicated serotypes including S. sonnei, S. flexneri, S. boydii, and S. dysenteriae are easily detected to help you identify potential contamination quickly.
Benefits
- Sensitivity and specificity consistent with the standard culture method for Shigella
- Closed-cap system avoids amplicon contamination in the lab
- LIMS-compatible electronic data for easy storage, sharing and retrieval
- Internal positive control included with each reaction
- Tableted PCR reagents provide product stability and longer shelf life
- Simplified sample prep and easy-to-follow prompts reduce training needs
- Real-time PCR processing complete in about one hour
- Automated DNA-based analysis provides clear yes-or-no results without the need for expert interpretation
Specifications
- Real-time
- Target: Shigella spp
- Enrichment time: 8-20 hours, depending on food type, sample volume and enrichment media
- PCR time: 60 minutes
- Detection level: 10^3 cfu/mL, after enrichment
Catalog No.
| Legacy Order Code | Hygiena Product Code | Description | Quantity |
| D14812018 | KIT2007 | BAX® System Real-time PCR Assay for Shigella | 96 tests per kit |
Applications & Validations
Validated Matrices for BAX® System Assays
The BAX® System holds the largest number of Validations and Approvals for PCR by third parties
Click here to download this full list in PDF format
Shigella |
||
|
Assay |
Approved Matrices |
Validation Reference |
|
BAX® System Real‐Time PCR Assay for Shigella KIT2007 (Kit #D14812018) |
Frankfurters | Internally Validated |
| Cooked meats, raw pork, dessert gelatin, ready‐to‐eat prepared dishes, non‐fermented soy products, lunch meals, granulated white sugar | Internally Validated |
Fit For Purpose Applications Data for BAX® System Shigella Assays
|
Sample Type |
Item |
Assays |
|
RTE |
Frankfurters |
Real-Time Shigella |
|
Cooked Meats |
||
|
Dessert Gelatin |
||
|
RTE prepared dishes |
||
|
Non-fermented soy products |
||
|
Lunch meals |
||
|
Granulated white sugar |
||
|
Hot Dog |
Real-Time Shigella |
|
|
Jelly |
||
|
Gongbao Chicken |
||
|
Herbs |
Parsley |
|
|
Cilantro |
||
|
Vegetable |
Sweet Pepper |
|
|
Spicy Pepper |
||
|
Mixed Salad |
||
|
Meat |
Pork |
|
|
Raw Pork |
Real-Time Shigella |
