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BAX® System Real-time PCR Assay for Shigella

Shigella naturally shares large portions of its' genetic sequence with many other pathogenic bacteria and is very easy to cross-contaminate with very lose dose response required. This cross-contamination primarily occurs from poor hygiene practices during food production and handling in retail environments. Utilizing the Real-Time PCR assay for Shigella can quickly eliminate negative samples and reduce presumptive positives seen in conventional methods due to the specificity and inclusivity of serotypes. Common implicated serotypes including S. sonnei, S. flexneri, S. boydii, and S. dysenteriae are easily detected to help you identify potential contamination quickly.

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 BAX Assay Kit2007 Shigella


Benefits

  • Sensitivity and specificity consistent with the standard culture method for Shigella
  • Closed-cap system avoids amplicon contamination in the lab
  • LIMS-compatible electronic data for easy storage, sharing and retrieval
  • Internal positive control included with each reaction
  • Tableted PCR reagents provide product stability and longer shelf life
  • Simplified sample prep and easy-to-follow prompts reduce training needs
  • Real-time PCR processing complete in about one hour
  • Automated DNA-based analysis provides clear yes-or-no results without the need for expert interpretation

Specifications

  • Real-time
  • Target: Shigella spp
  • Enrichment time: 8-20 hours, depending on food type, sample volume and enrichment media
  • PCR time:  60 minutes
  • Detection level:  10^3 cfu/mL, after enrichment

Catalog No.

Legacy Order Code  Hygiena Product Code Description Quantity
D14812018 KIT2007 BAX® System Real-time PCR Assay for Shigella  96 tests per kit

Applications & Validations

 Validated Matrices for BAX® System Assays

The BAX® System holds the largest number of Validations and Approvals for PCR by third parties

 Click here to download this full list in PDF format

Shigella

Assay

Approved Matrices

Validation Reference

 BAX® System Real‐Time PCR Assay for

Shigella

KIT2007 (Kit #D14812018)

 Frankfurters  Internally Validated
 Cooked meats, raw pork, dessert gelatin, ready‐to‐eat prepared dishes, non‐fermented soy products, lunch meals, granulated white sugar  Internally Validated

 Fit For Purpose Applications Data for BAX® System Shigella Assays

Sample Type

Item

Assays

RTE

Frankfurters

Real-Time Shigella

Cooked Meats

Dessert Gelatin

RTE prepared dishes

Non-fermented soy products

Lunch meals

Granulated white sugar

Hot Dog

Real-Time Shigella

Jelly

Gongbao Chicken

Herbs

Parsley

Cilantro

Vegetable

Sweet Pepper

Spicy Pepper

Mixed Salad

Meat

Pork

Raw Pork

Real-Time Shigella