Question 1

Are Hygiena gluten test kits AOAC certified?

Accepted Answer

Yes. GlutenTox kits use AOAC-certified G12 antibody technology, which targets the immunotoxic 33-mer gluten peptide for reliable, validated results.

Question 2

How do you test a surface for gluten after cleaning?

Accepted Answer

Swab the cleaned surface and run GlutenTox Pro Surface, which detects gluten residue down to 10 ng/cm² and gives a result in about 10 minutes. This verifies that changeover cleaning has removed gluten before the next production run.

Question 3

How do you test food for gluten?

Accepted Answer

Extract a small sample, add it to the test kit’s buffer, then run it on a lateral flow device for a rapid yes/no, or on an ELISA plate for a numeric ppm result. Hygiena’s GlutenTox Pro returns a result in under 20 minutes at a 5 ppm threshold.

Question 4

How long do gluten test results take?

Accepted Answer

Rapid lateral flow food tests take under 20 minutes, surface swab tests about 10 minutes, and quantitative ELISA around one to two hours depending on the protocol.

Question 5

What gluten level is considered gluten-free?

Accepted Answer

Both the U.S. FDA and Codex Alimentarius set the gluten-free limit at 20 ppm. To verify a gluten-free claim you need a quantitative method such as ELISA; GlutenTox ELISA Rapid G12 measures down to 0.4 ppm.

Question 6

What is a gluten test kit?

Accepted Answer

A gluten test kit detects gluten proteins from wheat, barley and rye in food, ingredients, rinse water or on surfaces. Kits range from rapid lateral flow strips that give a pass/fail result in minutes to quantitative ELISA assays that report an exact gluten level in ppm.

Question 7

What is the difference between lateral flow, ELISA and PCR gluten tests?

Accepted Answer

Lateral flow gives a fast pass/fail at the line, ELISA gives a documented ppm number for label verification, and PCR detects gluten DNA in complex or hydrolysed products. Most facilities screen with lateral flow and confirm with ELISA.

Question 8

Yes. GlutenTox kits use AOAC-certified G12 antibody technology, which targets the immunotoxic 33-mer gluten peptide for reliable, validated results.

Accepted Answer

Use a quantitative ELISA such as GlutenTox ELISA Rapid G12, which measures gluten down to 0.4 ppm and gives the documented ppm value auditors and regulators expect for a gluten-free claim.

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