Reagents for the DNA/RNA Extraction
See Reagents →Reagents and components for DNA/RNA extraction and real-time PCR for an optimal and robust analysis.
Reagent D eliminates DNA from dead cells to prevent false-positive PCR results in food safety testing. This light-sensitive reagent penetrates dead cell membranes and binds to DNA upon light exposure, blocking PCR amplification. Validated for testing Enterobacteriaceae, Cronobacter, Salmonella, Legionella, and Vibrio in various food matrices including instant milk formula and dairy products. Compatible with Hygiena's DNA isolation kits, Reagent D ensures accurate detection of viable microorganisms in food safety and environmental monitoring applications.
The foodproof Reagent P, designed for automated DNA isolation, works with foodproof Magnetic Preparation Kit I on RoboPrep Series workstations to extract gram-negative bacterial DNA from protein-rich food enrichment cultures. This specialized protein reagent optimizes cell lysis and DNA binding to magnetic beads, making it essential for processing samples like egg, ham, beef, pork, chicken, minced meat, salmon, and cheese. With 550 isolations per kit, it delivers high-quality DNA suitable for PCR applications, streamlining bacterial detection workflows in food safety testing laboratories.
Resuspension Reagent simplifies bacterial DNA preparation for PCR applications in food testing. Used with the foodproof StarPrep Two Kit, this specialized reagent minimizes inhibition. Designed for food safety laboratories and quality control facilities, it provides an efficient solution for microbial DNA extraction. This ready-to-use reagent helps food testing professionals maintain consistent and reliable PCR results while supporting their safety compliance requirements.
The Rinse Buffer is designed for optimal recovery of microbes from filter membranes.
The Suspension Buffer simplifies bacterial colony preparation for PCR testing with a streamlined, single-tube protocol. This specialized buffer enables direct suspension of colonies from agar plates without hazardous organic extractions or chaotropic agents. The extract maintains cell viability for additional analyses like serotyping or DNA sequencing. The efficient process delivers PCR-ready samples while reducing handling steps and exposure to hazardous materials. Perfect for quality control labs needing fast, reliable sample preparation for food safety and microbial identification testing.
BAX® System Free DNA Cleanup Kit effectively removes DNA not protected by living cells from food and environmental samples. This essential tool eliminates DNA from dead cells and bacteriophage intervention residuals, ensuring accurate pathogen detection results in food safety testing. Validated for poultry, poultry rinses, and environmental surface testing, this kit seamlessly integrates with BAX® System methods to support reliable PCR-based testing procedures. The simple protocol requires minimal hands-on time and delivers consistent results for quality assurance testing.
Components for Real-Time PCR
See Components →Additional components for foodproof® and microproof® Real-Time PCR kits.
The Internal Amplification Control serves as a control template for colony confirmation procedures in real-time PCR testing with Hygiena's LyoKits. This versatile control is compatible with multiple detection kits, including those for Salmonella, STEC, Vibrio, and Legionella testing. Designed specifically for shortened PCR cycles, it ensures reliable results in colony confirmation workflows. The control enhances testing accuracy across food safety and environmental monitoring applications, making it an essential component for quality assurance protocols.
This reference material is used for the quantitative analysis of allergens in food samples. It contains celery, soy, peanut, hazelnut, walnut, sesame and gluten in a defined concentration of 800 mg/kg.
Color Compensation Set 1 is a calibration solution designed for use with LightCycler® Systems in food safety testing applications. This set offers precise color compensation for fluorescence detection across multiple channels, enabling accurate analysis of hybridization probe-based assays. Compatible with LightCycler® 480 Systems, the kit supports 5 calibration runs and is particularly suited for the analysis of Enterobacteriaceae plus Cronobacter Detection Systems. This reliable calibration tool ensures optimal fluorescence detection and cross-talk compensation for dependable food safety testing results.
The Color Compensation Set 3 from Hygiena is designed for accurate color compensation calibration on the LightCycler® System. This essential calibration tool enables precise multicolor experiments across various food safety testing applications, including GMO detection, pathogen screening, and virus detection. The kit provides calibrators for four fluorescent channels (FAM, HEX, ROX, Cy5) and generates instrument-specific color compensation objects for reliable analysis of multiple Hygiena real-time PCR assays. Suitable for 5 calibration runs, it ensures accurate results in food safety testing laboratories.
The Color Compensation Set 5 is designed for creating color compensation objects for the LightCycler® 480 System, enabling accurate multicolor experiments in molecular testing. This essential calibration tool supports various foodproof® detection kits for pathogens like Yersinia enterocolitica, Vibrio, Salmonella, and Cronobacter, as well as spoilage yeasts and Aspergillus. Each set provides materials for 5 calibration runs using four different fluorophores (FAM, HEX, ROX, and Cy5), ensuring precise detection across multiple channels.
Other Reagents
See Other Reagents →Further products that are related to other product groups.
ZymoSnap Positive Control Kit
The Positive Control Kit for ZymoSnap ALP validates the performance of ZymoSnap ALP test devices for measuring alkaline phosphatase in pasteurized milk products. Containing five vials of 350 mU/L ALP concentration, this kit helps establish and verify fail RLU levels for different milk types (4%, 2%, and 0.1%). Using heat-inactivated milk samples as negative controls, the kit supports quality control programs by confirming test device functionality and maintaining accurate detection of ALP at regulatory levels. Compatible with EnSURE Touch and EnSURE monitoring systems, it ensures reliable pasteurization verification testing.