Fast and Reliable Real-Time PCR E. coli Detection
From raw materials to finished products, our E. coli real-time PCR testing kits ensure food safety at every step. Backed by AOAC, MicroVal, and AFNOR certifications, these validated solutions combine high-throughput capabilities with proven accuracy, supporting your quality assurance programs with reliable results.
E. coli Testing: Ensuring Food Safety and Brand Trust
Escherichia coli O157:H7 is a dangerous foodborne pathogen that poses a significant threat to public health. Each year in the United States, an estimated 73,480 illnesses occur due to E. coli O157 infections, resulting in approximately 2,168 hospitalizations and 61 deaths. This bacterium is particularly concerning as it can cause severe complications, including hemolytic uremic syndrome, which is a leading cause of acute renal failure in children (NIH). In September 2024, a significant outbreak affected 104 people across 14 states, demonstrating the ongoing challenge this pathogen presents to food safety systems (CDC).
The food industry faces substantial challenges in preventing E. coli contamination, with ground beef being the most frequently identified vehicle in 41% of foodborne outbreaks. Other sources such as produce, dairy products, and even person-to-person transmission in settings like daycare centers also contribute to the spread of this pathogen (NIH). The global E. coli testing market, valued at $2.1 billion in 2023, is projected to reach $3.9 billion by 2033, reflecting the growing importance of rapid and reliable detection methods in safeguarding public health (Allied Market Research).
Given the significant health and economic impacts of E. coli outbreaks, regular testing is crucial for ensuring food safety.
The Most Important Facts About: E. coli
- As few as 10 E. coli O157:H7 cells can cause severe illness, making highly sensitive detection critical for food safety.
- While E. coli is killed at 70°C (158°F), it survives freezing and grows rapidly between 10-40°C (50-104°F), requiring strict temperature control.
- E. coli can transfer between food contact surfaces for up to 8 hours, highlighting the importance of robust sanitation programs.
- Major E. coli recalls typically cost food companies $1.5-2.5 million, not including long-term brand damage.
- E. coli O157:H7 cases lead to about 2,000 hospitalizations annually in the US, with meat, dairy, and produce being common sources.
Benefits of (Real-Time) PCR E. coli Detection
PCR testing identifies specific E. coli DNA sequences, precisely detecting O157:H7 and other strains in food samples. This method provides several technical advantages compared to traditional culture-based approaches.
- Detection limits of ≤10 CFU/25g for E. coli O157:H7 ensure compliance with zero-tolerance policies in high-risk foods like ground beef and ready-to-eat products
- Specific DNA-based identification of pathogenic E. coli strains eliminates false positives from non-pathogenic E. coli, reducing unnecessary product holds
- Complete testing protocol in 8-24 hours (including enrichment) versus 4-7 days for culture methods, enabling faster product release decisions
For more information about PCR and real-time PCR, visit our Technology Guide.
Our Proven E. coli PCR Detection Solutions
Select from our range of validated PCR and real-time PCR solutions for E. coli and Shigella detection, with options for O157:H7. Filter by certification (AOAC, MicroVal, AFNOR) or brand to find the right testing solution for your laboratory.
Filter products:
Filter products:
Not found what you are looking for?
Related Products for E. coli PCR Testing
Browse our instruments and accessories designed for reliable E. coli PCR testing workflows. From DNA extraction systems and thermal cyclers to automated platforms, these validated tools ensure consistent results across your testing protocols.
Rapid E. coli Screening for Environmental Monitoring
Complement your PCR testing with ATP-based screening solutions for quick E. coli monitoring. These tools provide immediate indication of microbial presence, supporting your environmental monitoring program and pre-screening needs.
FAQs
Find answers to common technical questions about E. coli PCR detection methods, sample preparation, and result interpretation. Our technical support team regularly updates these FAQs based on laboratory feedback and testing requirements.