
2021 IAFP Scientific Posters
2021 IAFP Sessions and Technical Presentations
S8 Reducing Food Safety Risks of Pork Products: Science-based and Data-driven Steps to reduce Salmonella
Sub-session: Principles behind using Real-Time PCR for Quantification of Salmonella Matrices
Location: Starts at 30:12
Presenter: Tyler Stephens, PhD – Government & Regulatory Affairs Manager
S23 Your Significant Other: Using Statistics to Interpret Microbiological Data
Presenter: Julie Weller – Applications Manager
S44 Beyond Metagenomic Sequencing: Metadata, Ontologies, and Big Data
Location: 129 A
Presenter: Pushpinder Litt – Senior R&D Scientist
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S35 We Quantified, Now What? Actual Salmonella Quantification Approaches Utilized in the Protein Industry Today
Conveners: Stacy Stoltenberg & April Englishbey
T11-03 Verification of Quantification and Limits Testing of Salmonella in Finely Textured Beef (FTB) Using Hygiena’s BAX® System Real-Time PCR Assay for Salmonella
Location: 132 AB
Presenter: April Englishbey
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ATP Monitoring and Cleaning Verification

Demonstration of Hygiena’s High Sensitivity SuperSnap™ ATP Surface Monitoring System as an Excellent Proxy Alternative Method for Remediation of True Allergens from Surfaces During Cleaning Verification
Location: P1-76
Presenter: Delia Calderon
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Mycotoxins

Application of a Universal ELISA Method to Detect Aflatoxin B1 in Diverse Commodities with Optimized Extraction Procedures
Location: P1-82
Presenter: Byungchul Kim
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Pathogen Testing & Detection

Performance Evaluation of Hygiena’s BAX® System for E. coli O157:H7 in Tree Nuts
Location: P1-90
Presenter: Victoria Kuhnel
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Detection of Salmonella in Garlic Powder using the Hygiena™ BAX® System
Location: P1-108
Presenter: Julie Weller
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Bio-Mapping of Pathogens and Indicator Organisms throughout the Poultry Processing Chain Using Hygiena’s MicroSnap™ and BAX® System SalQuant™, and bioMérieux Tempo® Methods
Location: P1-123
Presenter: Savannah Applegate
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Salmonella Quantification (SalQuant™) with Hygiena’s BAX® System for Ground Turkey
Location: P1-124
Presenter: Julie Weller
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Salmonella Quantification of Various Pork Matrices Utilizing Hygiena’s BAX® System SalQuant™
Location: P1-133
Presenter: Stacy Stoltenberg
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Using a Limits Approach to Detect Specific Levels of Salmonella in Beef Trim and MicroTally™ Swabs with Hygiena’s BAX® System
Location: P1-141
Presenter: Julie Weller
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Evaluation of Hygiena’s New BAX® System Real-Time PCR Assay for E. coli O157:H7 Exact in Meat, Produce and Raw Dairy Matrices
Location: P2-43
Presenter: Victoria Kuhnel
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Comparative Evaluation of BAX® Real-Time PCR Assay for the Detection of Shiga toxin E. coli (STEC) against the ISO/TS 13136 Reference Method
Location: P2-55
Presenter: Nisha Corrigan
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Internal Validation of Hygiena’s BAX® System for E. coli O157:H7 and Salmonella from Ready-to-Eat Chicken Sausage
Location: P2-44
Presenter: Victoria Kuhnel
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Evaluation of Hygiena’s BAX® System Real-Time PCR Assays for the Detection of E. coli O157:H7 from Mixed Sprouts
Location: P2-135
Presenter: Julie Weller
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Evaluation of the Hygiena™ BAX® System Real-Time PCR Assay for Salmonella in Poultry Primary Production Boot Swabs
Location: P2-116
Presenter: Julie Weller
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Detection of Salmonella in Whole Fresh Peaches using Hygiena’s BAX® System
Location: P2-159
Presenter: Victoria Kuhnel
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Evaluation of Hygiena’s BAX® System PCR Assays for the Detection of Salmonella from 375 g Frozen Potatoes
Location: P2-134
Presenter: Priyanka Surwade
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Method Validation of Shelled Pecans for Salmonella and Listeria monocytogenes using Hygiena’s BAX® System
Location: P2-39
Presenter: Julie Weller
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Evaluation of Hygiena’s BAX® System for the Detection of Salmonella and L. monocytogenes in Plant-Based Meat Substitutes
Location: P2-38
Presenter: Julie Weller
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Analysis of Individual and Pooled Environmental Samples using Hygiena’s BAX® System PCR Assays for Genus Listeria and L. monocytogenes
Location: P2-45
Presenter: Victoria Kuhnel
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Determination of Detection Limits of a Commercial RT-PCR for Campylobacter jejuni in Poultry Rinsates
Location: P2-37
Presenter: Aaron Bodie (University of Wisconsin-Madison)
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Quantification of Campylobacter jejuni in Poultry Processing Rinses Utilizing Shortened Enrichment Times and RT-PCR
Location: P-36
Presenter: Aaron Bodie (University of Wisconsin-Madison)
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Detection of Listeria monocytogenes in Mixed Environmental Sponge Swab Enrichment Cultures Using Hygiena™ BAX® PCR and Real-Time PCR Assays versus USDA MLG and FDA BAM Reference Methods
Location: P3-140
Presenter: Ryan Zimmerman (Deibel Laboratories, Inc.)
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Pathogen Quantification

Quantification of Campylobacter jejuni in Poultry Processing Rinses Utilizing Shortened Enrichment Times and RT-PCR
Location: P2-36
Presenter: Aaron Bodie / Authors: Savannah Applegate, Tyler Stephens, Steven Ricke
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Salmonella Quantification (SalQuant™) with Hygiena’s BAX® System for Turkey Feet Swabs and Cloacal Swabs
Location: P2-117
Presenter: Julie Weller
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Hygiena™ BAX® System SalQuant™ AOAC Validation for Comminuted Chicken and Turkey
Location: P3-130
Presenter: Tyler Stephens
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Indicator Organism Testing & Detection

Quantitation of Listeria spp from Surfaces Using New Rapid Bioluminogenic Detection Swabs and EnSURE™ Touch Luminometer from Hygiena™
Location: P2-168
Presenter: Delia Calderon
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Hygiena’s MicroSnapTM Total Viable Count System Correlation with Standard Methods from Eighty-Eight Different Food Matrices Including Dairy, Protein, and Water and Miscellaneous
Location: P2-53
Presenter: Delia Calderon
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Microbial Screening

Advantages of Rapid ATP Sterility Testing using Hygiena’s Innovate System for UHT and ESL Alternative Milk-Based Products Compared to pH and Standard Plating
Location: P2-48
Presenter: Rafael Barajas
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