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How PCR Works

how pcr worksInside the BAX® System instrument, prepared samples undergo a series of heating and cooling cycles. Heating denatures the DNA, separating it into single strands. As the mixture cools, primers recognize and anneal (bind) to the targeted DNA sequence. Polymerase then uses nucleotides to extend the primers, creating two copies of the targeted DNA fragment (amplification). Repeating the cycle of denaturing, annealing and amplification produces an exponential increase in the number of DNA fragments, creating millions of copies in a very short time. If the target sequence is not 

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