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Food Safety/
Pathogen Detection/
STEC

foodproof® STEC Screening LyoKit - Real-Time PCR for STEC Detection in Beef

The foodproof® STEC Screening LyoKit delivers rapid, reliable STEC detection for beef and meat testing laboratories. This AOAC-certified real-time PCR assay simultaneously screens for stx1, stx2, and eae virulence genes in a single triplex reaction, enabling comprehensive Shiga toxin-producing E. coli identification. Validated for fresh raw ground beef and beef trim with an 8-hour enrichment protocol, laboratories achieve same-day results with less than 40 minutes hands-on time. The lyophilized format ensures consistent performance and simplified workflows, while ISO/TS 13136 compliance and 100% inclusivity across 238 STEC strains provide the analytical confidence food safety professionals require.

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The foodproof® STEC Screening LyoKit delivers rapid, reliable STEC detection for beef and meat testing laboratories. This AOAC-certified real-time PCR assay simultaneously screens for stx1, stx2, and eae virulence genes in a single triplex reaction, enabling comprehensive Shiga toxin-producing E. coli identification. Validated for fresh raw ground beef and beef trim with an 8-hour enrichment protocol, laboratories achieve same-day results with less than 40 minutes hands-on time. The lyophilized format ensures consistent performance and simplified workflows, while ISO/TS 13136 compliance and 100% inclusivity across 238 STEC strains provide the analytical confidence food safety professionals require.

eae,

stx

, stx1, stx2
Technology: Real-time PCR
AOAC-RI
LP: KIT230077
RP: KIT230078

Key Benefits

Easy-to-Use
Few pipetting steps
Simple sample and reagent handling
Superior Sensitivity
Detects small organism numbers with low DNA levels (or low levels of DNA)
Easily detects low-level contamination, keeping affected food from being released and decreasing risk of recalls
Rapid Results
Reduced time to results enables faster decisions
Dozens of protocols to ensure faster detection/quantitation for your specific matrix of concern
Proven Protocols
Validated methods across many applications & matrices
Specific methods for the most commonly affected matrices
Intuitive Software
Accurate algorithms for result analysis and interpretation
Providing in-depth insights.
Broad Compatibility
Suitable for a variety of real-time cyclers
Allows fast integration into existing PCR labs

foodproof® Workflow

The foodproof® workflow delivers highly reliable detection through a systematic three-step approach: enrichment, optimized DNA extraction matched to your sample type and throughput needs, and real-time PCR detection. This standardized process ensures superior accuracy through high-purity DNA extracts, making it ideal for challenging food matrices.

If you are using a Roche LightCycler® 480 with this detection kit use the Color Compensation Set 3.
1
Sample Enrichment
Specific growth of target organisms via selective media for a reliable detection
2
Tailored DNA/RNA Extraction
Procedures tailored to your needs to always ensure a high-quality DNA extraction even from complex samples matrices
3
Real-Time PCR Detection
Assay execution and real-time PCR amplification for clear presence/absence results

Buy This Product Online

Ready to order? Purchase this product directly from the US eStore or Australia eStore. This product may be available in different variations and sizes.
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foodproof ®

STEC Screening LyoKit - Real-Time PCR for STEC Detection in Beef

LP
KIT230077

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