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Food Safety/
Pathogen Detection/
Vibrio

foodproof® Vibrio Detection LyoKit

The foodproof Vibrio Detection LyoKit offers reliable detection of Vibrio parahaemolyticus, V. vulnificus, and V. cholerae in seafood and environmental samples using real-time PCR technology. This AOAC-validated kit enables identification of pathogenicity-associated toxin genes tdh, trh1, trh2, and ctx through melting curve analysis. Designed for 96 reactions with ready-to-use lyophilized PCR mix, it delivers accurate results from enriched cultures within hours. Compatible with major real-time PCR platforms, this kit brings streamlined Vibrio testing to food safety laboratories.

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The foodproof Vibrio Detection LyoKit offers reliable detection of Vibrio parahaemolyticus, V. vulnificus, and V. cholerae in seafood and environmental samples using real-time PCR technology. This AOAC-validated kit enables identification of pathogenicity-associated toxin genes tdh, trh1, trh2, and ctx through melting curve analysis. Designed for 96 reactions with ready-to-use lyophilized PCR mix, it delivers accurate results from enriched cultures within hours. Compatible with major real-time PCR platforms, this kit brings streamlined Vibrio testing to food safety laboratories.

V. cholerae, V. parahaemolyticus, V. vulnificus, toxin genes (tdh, trh1, trh2, ctx)
AOAC-RI
96
KIT230117 (LP), KIT230118 (RP)

Key Benefits

Easy to Use
Streamlined workflows with simplified steps
Minimizes user errors and cross-contamination.
Superior Sensitivity
Detects small organism numbers with low DNA levels (or low levels of DNA)
Easily detects low-level contamination, keeping affected food from being released and decreasing risk of recalls.
Rapid Results
Reduced time to results enables faster decisions
Dozens of protocols to ensure faster detection/quantitation for your specific matrix of concern.
Proven Protocols
Validated methods across many applications & matrices
Specific methods for the most commonly affected matrices.
Intuitive Software
Accurate algorithms for result analysis and interpretation
Retrieving data. Wait a few seconds and try to cut or copy again.
Broad Compatibility
Suitable for a variety of real-time cyclers
Allows fast integration into an already existing PCR lab

foodproof® Workflow

We offer a broad portfolio of products for a robust and reliable real-time PCR detection by providing not only kits tailored to the needs in your lab but also instruments for automation of liquid handling and real-time PCR cyclers.

Sample Enrichment
Workflow next
Tailored DNA/RNA Extraction
Workflow next
Real-Time PCR Detection
If you are using a Roche LightCycler® 480 with this detection kit use the Color Compensation Set 5.

Real-Time PCR Instruments

Our foodproof and microproof PCR kits are compatible with many real-time PCR cyclers. For an overview reference the product sheet or contact us.

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 Dualo 32® R2

The Dualo 32 R2 is a robust 32-well instrument in a convenient format, ideal for facilities with small to medium throughput and little lab space. The wide optical spectrum allows the flexible use of both hydrolysis and hybridization probes. The instrument is optimized for quality control for all different parameters in the food and feed industry.

MCH230003
Product Sheet
Product Instructions
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Extraction

We provide extraction kits tailored to your needs:

Extraction Type:?
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foodproof® StarPrep Three Kit

The foodproof StarPrep Three Kit enables rapid DNA extraction from Gram-negative bacteria for PCR testing. This efficient extraction method delivers PCR-ready DNA in under 30 minutes from enrichment cultures, direct samples, and filtered samples. The single-tube protocol eliminates hazardous organic extractions and minimizes cross-contamination risks. Designed for up to 400 reactions, this kit offers streamlined DNA preparation with fewer handling steps compared to traditional methods, making it an essential tool for quality control laboratories.

21 mL
KIT230187
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Depending on the extraction protocol, additional reagents and devices are used:

Additional Reagents

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 Reagent D

Reagent D eliminates DNA from dead cells to prevent false-positive PCR results in food safety testing. This light-sensitive reagent penetrates dead cell membranes and binds to DNA upon light exposure, blocking PCR amplification. Validated for testing Enterobacteriaceae, Cronobacter, Salmonella, Legionella, and Vibrio in various food matrices including instant milk formula and dairy products. Compatible with Hygiena's DNA isolation kits, Reagent D ensures accurate detection of viable microorganisms in food safety and environmental monitoring applications.

KIT230001
Product Instructions
Reference Guide
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Product Library

Filter documents:
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Categories
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Bringing the Power of PCR to Food Safety
White PaperEnglish
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Food Safety in the Beef Industry
White PaperEnglish
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foodproof Vibrio Detection Kits Product Sheet (EN)
Product SheetEnglish
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foodproof Vibrio Detection LyoKit Product Instructions
InstructionsEnglish
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foodproof Vibrio Detection LyoKit Ready Reference Guide
Ready Reference GuidesEnglish
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foodproof® Clostridium botulinum Detection LyoKit SDS (EN)
Safety Data SheetEnglish
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PCR vs ELISA In-Process Test Selection for the Food Industry
White PaperEnglish
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Recalls & PCR Results Interpretation - PCR Software that Protects Your Brand
White PaperEnglish
8 Documents