Highly Specific and Sensitive Allergen Detection
AlerTox® ELISA is a quantitative immunosorbent assay designed for the quantitative determination of allergens in raw materials and final products. The ELISA Sandwich is a technique usually used for the analysis of substances found at very low concentrations. This combined with the high specificity and sensitivity of the antibodies used in these tests allows this method to precisely quantify allergens in all types of food and drinks.
Key Benefits
Highly Senstive
Highly Specific
Fast Results
20 Allergens
Easy-to-use
All AlerTox® ELISA tests work on the principle of a quantitative sandwich ELISA. An antibody directed against the target antigen (protein) is bound on the surface of a microtiter plate. Antigen-containing samples or standards are given into the wells of the microtiter plate. After a 20 minute incubation at room temperature (15-25º C/59-77º F), the wells are washed with diluted washing solution to remove unbound material.
A peroxidase-conjugated second antibody directed against the same antigen is given into the wells and after 20 minutes of incubation, the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a Stop Solution, and the color turns yellow. The yellow color is measured photometrically at 450 nm. The concentration of the antigen is directly proportional to the color intensity of the test sample.
Limits of Detection and Quantification
Proven Performance
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How to Use
| Kit | LOD | LOQ |
| AlerTox® ELISA Almond | 0.2 ppm | 0.5 ppm |
| AlerTox® ELISA Beta-lactoglobulin | 1.5 ppb | 10 ppb |
| AlerTox® ELISA Casein | 0.05 ppm | 0.2 ppm |
| AlerTox® ELISA Cashew | 0.02 ppm | 2 ppm |
| AlerTox® ELISA Coconut | 0.4 ppm | 2 ppm |
| AlerTox® ELISA Crustacean | 1 ppb | 20 ppb |
| AlerTox® ELISA Egg (egg white proteins) | 0.05 ppm | 0.4 ppm |
| AlerTox® ELISA Fish | 1.4 ppm | 4 ppm |
| AlerTox® ELISA Hazelnut | 0.3 ppm | 1 ppm |
| AlerTox® ELISA Lupine | 0.2 ppm | 2 ppm |
| AlerTox® ELISA Lysozyme | 2 ppb | 25 ppb |
| AlerTox® ELISA Macadamia | 0.1 ppm | 1 ppm |
| AlerTox® ELISA Milk (Casein & Beta-lactoglobulin) | 0.05 ppm | 0.5 ppm |
| AlerTox® ELISA Mustard (white, black, brown) | 1.5 ppm | 2 ppm |
| AlerTox® ELISA Ovalbumin | 5 ppb | 25 ppb |
| AlerTox® ELISA Peanut | 0.3 ppm | 1 ppm |
| AlerTox® ELISA Pistachio | 0.13 ppm | 1 ppm |
| AlerTox® ELISA Sesame | 0.2 ppm | 2 ppm |
| AlerTox® ELISA Soy (STI=Soy Trypsin Inhibitor) | 16 ppb | 50 ppb |
| AlerTox® ELISA Walnut | 0.6 ppm | 2 ppm |
| Catalog No. | D-Code | Description | Quantity | Contact Sales |
| KIT3049 | AlerTox® ELISA Almond | 96 well kit | Learn More | |
| KIT3042 | AlerTox® ELISA Beta-lactoglobuline | 96 well kit | Learn More | |
| KIT3043 | AlerTox® ELISA Casein | 96 well kit | Learn More | |
| KIT3053 | AlerTox® ELISA Cashew | 96 well kit | Learn More | |
| KIT3056 | AlerTox® ELISA Coconut | 96 well kit | Learn More | |
| KIT3059 | AlerTox® ELISA Crustacean | 96 well kit | Learn More | |
| KIT3046 | AlerTox® ELISA Egg (Egg white proteins) | 96 well kit | Learn More | |
| KIT3060 | AlerTox® ELISA Fish | 96 well kit | Learn More | |
| KIT3053 | AlerTox® ELISA Hazelnut | 96 well kit | Learn More | |
| KIT3057 | AlerTox® ELISA Lupine | 96 well kit | Learn More | |
| KIT3044 | AlerTox® ELISA Lysozyme | 96 well kit | Learn More | |
| KIT3055 | AlerTox® ELISA Macadamia | 96 well kit | Learn More | |
| KIT3041 | AlerTox® ELISA Milk (Casein & Beta-lactoglobulin) | 96 well kit | Learn More | |
| KIT3058 | AlerTox® ELISA Mustard (white, black, brown) | 96 well kit | Learn More | |
| KIT3045 | AlerTox® ELISA Ovalbumin | 96 well kit | Learn More | |
| KIT3048 | AlerTox® ELISA Peanut | 96 well kit | Learn More | |
| KIT3054 | AlerTox® ELISA Pistachio | 96 well kit | Learn More | |
| KIT3051 | AlerTox® ELISA Sesame | 96 well kit | Learn More | |
| KIT3047 | AlerTox® ELISA Soy (STI=Soy Trypsin Inhibitor) | 96 well kit | Learn More | |
| KIT3052 | AlerTox® ELISA Walnut | 96 well kit | Learn More |
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