microproof® Legionella Quantification LyoKit - Real-Time PCR Testing for Water Quality

Reagent D is a viability PCR reagent engineered for dead cell DNA elimination in food safety testing. This photoactive solution enables live/dead cell differentiation by penetrating compromised cell membranes and cross-linking with DNA upon light exposure, effectively blocking PCR amplification of non-viable organisms for reliable false positive prevention. Featuring a rapid 10-minute workflow, Reagent D is validated for Enterobacteriaceae, Cronobacter, Salmonella, Vibrio, Yeast/Mold, and Legionella detection. Compatible with StarPrep® and LyoKit extraction systems, it delivers accurate results in complex matrices including dairy products, infant cereals, and environmental water samples.

The Rinse Buffer is a ready-to-use DNA extraction rinse solution designed for efficient microorganism recovery from filter membrane surfaces. Optimized for membrane filtration workflows, this standardized reagent integrates seamlessly with the foodproof® StarPrep® Two Kit protocol. It supports validated methods for Legionella water testing and spoilage yeast detection in environmental samples. The 100 mL bottle format ensures consistent results across laboratory shifts and locations, eliminating variability from lab-prepared buffers. By maximizing cell recovery from filter matrices, laboratories achieve reliable pathogen detection with reduced preparation time.

The Suspension Buffer streamlines bacterial cell suspension preparation for real-time PCR testing in food safety laboratories. This non-lytic reagent enables direct suspension of colonies from agar plates, delivering PCR-ready DNA samples without hazardous organic extractions. The buffer preserves cell viability, allowing downstream confirmatory testing such as serotyping or DNA sequencing from a single sample. Validated for use with foodproof®, microproof®, and vetproof® detection kits, it supports both single-tube protocols and high-throughput 96-well plate workflows. Ambient storage at 15-25C simplifies inventory management in QC laboratories.