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Food Safety/
Pathogen Detection/
STEC

BAX® System Real-Time STEC Panel 2 - PCR Detection for E. coli O45, O103, O145

The BAX® System Real-Time STEC Panel 2 delivers rapid, accurate non-O157 STEC detection for Shiga toxin-producing E. coli serogroups O45, O103, and O145—USDA-declared adulterants in raw beef and other food matrices. Designed for beef and dairy processors, this confirmatory PCR assay works seamlessly with the BAX® STEC Screening kit, using the same lysate to eliminate redundant sample preparation. With AOAC-RI and AFNOR certification, results in as little as 9 hours, and validated performance across raw beef, dairy, vegetables, and cannabis, it supports regulatory compliance and accelerates product release decisions.

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The BAX® System Real-Time STEC Panel 2 delivers rapid, accurate non-O157 STEC detection for Shiga toxin-producing E. coli serogroups O45, O103, and O145—USDA-declared adulterants in raw beef and other food matrices. Designed for beef and dairy processors, this confirmatory PCR assay works seamlessly with the BAX® STEC Screening kit, using the same lysate to eliminate redundant sample preparation. With AOAC-RI and AFNOR certification, results in as little as 9 hours, and validated performance across raw beef, dairy, vegetables, and cannabis, it supports regulatory compliance and accelerates product release decisions.

O103, O145, O45
Technology: Real-time PCR
AOAC-RI, AFNOR (STEC Suite & E. coli), AFNOR (STEC Suite & E. coli, French), USDA-FSIS (MLG 5B.05 adoption)
KIT2009

Key Benefits

Accurate Results
Trust results due to a high sensitivity and specificity
Differentiates closely related species, meaning fewer re-tests and less waste of truly safe food
Easy-to-Use
Few pipetting steps
Simple sample and reagent handling
Rapid Results
Reduced time to results enables faster decisions
Dozens of protocols to ensure faster detection/quantitation for your specific matrix of concern
Simplified Workflow
Increase lab productivity with streamlined protocols
Less hands-on time frees technician time for other lab work
Proven Protocols
Validated methods across many applications & matrices
Specific methods for the most commonly affected matrices
Intuitive Software
Accurate algorithms for result analysis and interpretation
Providing in-depth insights.

BAX® System Workflow

The BAX® System PCR workflow combines simple enrichment with convenient (real-time) PCR testing, delivering highly accurate pathogen detection results in a standardized format. As a complete solution, it includes all necessary instruments, software, and reagents for consistent and reliable testing across your facilities.

1
Sample Enrichment
Specific growth of target organisms via selective media for a reliable detection
2
Lysis and Real-Time PCR Detection
Assay execution and (real-time) PCR amplification for clear presence/absence results

Automation Solutions

Hygiena Prep Xpress
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Hygiena Prep Xpress

Hygiena® Prep Xpress - Automated Liquid Handling Platform for Food Safety Laboratories

The Hygiena Prep Xpress is a compact automated liquid handling system designed for food safety lab automation and high-throughput pathogen detection workflows. This versatile pipetting station eliminates manual errors and reduces technician fatigue during BAX System sample preparation, including reagent dispensing, serial dilutions, and PCR setup automation. Featuring air displacement pipetting with 1.0% CV accuracy, intelligent liquid level detection, and an integrated camera for verified deck loading, the Prep Xpress delivers consistent, reproducible results. Its flexible configuration supports two independent channels or an 8-probe head to match fluctuating sample volumes, while user-replaceable components minimize downtime.

MCH2075
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Product Library

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Alternate Storage Procedure for BAX Lysis Reagent in Cluster Tube Racks
Technical BulletinEnglish
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BAX Detection for E. coli in Sprouts
Technical BulletinEnglish
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BAX IAFP Regulatory Updates Accepted Use of Rapid Microbial Methods
White PaperEnglish
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BAX System PCR Internal Positive Control
Technical BulletinEnglish
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BAX System PCR Validated Matrices
CertificationEnglish
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BAX System Q7 Brochure (EN)
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BAX System Q7 Real-time PCR Assay Ready Reference Guide (DE)
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33 Documents

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BAX® System

Real-Time STEC Panel 2 - PCR Detection for E. coli O45, O103, O145

KIT2009

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