The AlerTox® ELISA Walnut is a quantitative walnut allergen test kit designed for food safety professionals in confectionary, dairy, and prepared foods manufacturing. This tree nut ELISA kit quantifies walnut proteins from 2 to 50 ppm with a 0.4 ppm detection limit, supporting allergen control programs and labeling compliance. The assay delivers exceptional specificity with zero cross-reactivity against Brazil nut, cashew, macadamia, and peanut. A 60-minute incubation protocol and universal extraction method compatible with 16 AlerTox® assays streamline food allergen testing workflows. FAPAS validated across diverse matrices including chocolate, cookies, ice cream, and baked goods.